چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Herein, a surface plasmon resonance (SPR) enhanced DNA biosensor has been developed for real-time detection of donkey meat marker using biotinylated reporter and streptavidin functionalized gold nanostars (Stre@GNSs). Compared to the direct detection assay, this sandwich format for the enhancement of the signal, resulted in 6-folds orders increase in the sensitivity. Target DNA could be detected with the lower limit of quantification (LLOQ) of 1.0 nM with a relative standard deviation (RSD, n = 3) of 0.85%. In addition, the fabricated SPR sensor showed good selectivity for the target analyte over full complementary, single-base mismatch, three basemismatch and non-complementary oligonucleotides. Finally, the proposed sensor was successfully applied for detection of donkey meat adulteration with various percentages in homemade beef sausage, as a real sample. The results indicated that the proposed biosensor provides a high specificity, easy, good sensitivity and fast approach for identification of donkey meat adulteration in food samples. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمدرضا رشیدی شاهگلی | هفتم |
| مریم منصوری | اول |
| سیاوش دستمالچی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| SPR enhanced DNA biosensor for sensitive detection of donkey meat adulteration.pdf | 1400/08/22 | 1603348 | دانلود |
