چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| The reports show that rutin has good potentials as an anticancer agent; however, rutin has poor bioavailability due to its low aqueous solubility. The present study was aimed at preparing and evaluating physicochemical properties as well as the anticancer activities of rutin nanocrystals (RNs). RNs were prepared via the ultrasonication method. The prepared nanocrystals then were physicochemically characterized by the conventional techniques. The cytotoxic effect of RNs and free rutin on the HN5 head and neck squamous carcinoma cell line was assessed. The HGF1-PI1 cells as normal oral cells were treated by RNs. Cells were also exposed to rutin and RNs to determine their effects on the expression of caspase-8, caspase-9, Bcl-2, and Bax genes. The prepared RNs have a mean particle size of nm and quasispherical morphology. Rutin displayed no significant cytotoxic effect on HN5 cells to 2000 μM. However, RNs displayed a cytotoxic effect with IC50 of 30.51 μM and 27.34 μM in 24 and 48 h incubation times, respectively (p<0.05). RNs had cytotoxic effect 100 times more than rutin on HN5 cells. There was no significant cytotoxic effect on HGF1-PI1 treated by RNs in 24 and 48 h. The expression of Bcl-2 mRNA was significantly decreased in attendance of RNs compared to the control group (p<0.05). The increase in Bax/Bcl-2 ratio was revealed within IC50 of RNs in 24 h. Our results confirm that the anticancer effect of RNs is significantly more than that of rutin. The activation of the mitochondria-dependent apoptotic pathway of RNs occurred via modulation of Bcl-2 and Bax expression. These results suggest that RNs may be useful in the development of a cancer therapy protocol. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سپیده بهلولی | اول |
| فائزه جعفرمدار قره باغ | دوم |
| الهه دلیرعبدالهی نیا | سوم |
| مریم کوه سلطانی | چهارم |
| قاسم ابراهیمی | پنجم |
| لیلا روشنگر | ششم |
| امیر ایمانی | هفتم |
| سیمین شریفی | هشتم |
| سولماز ملکی دیزج | نهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 9980451.pdf | 1400/09/03 | 936823 | دانلود |
