چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | هانیه صالحی پورمهر |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات پزشکی مبتنی بر شواهد EBM |
| کد مقاله | 76733 |
| عنوان فارسی مقاله | Mesenchymal stem cells injection attenuates spinal cord injury-induced neurogenic bladder via modulation of myogenesis, angiogenesis, trophic factors and apoptosis in female rats |
| عنوان لاتین مقاله | Mesenchymal stem cells injection attenuates spinal cord injury-induced neurogenic bladder via modulation of myogenesis, angiogenesis, trophic factors and apoptosis in female rats |
| نوع ارائه | پوسترو سخنرانی |
| عنوان کنگره / همایش | European association of urology21 |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Netherlands |
| شهر محل برگزاری کنگره/ همایش | virtual |
| سال انتشار/ ارائه شمسی | 1400 |
| سال انتشار/ارائه میلادی | 2021 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1400/04/18 الی 1400/04/22 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.sciencedirect.com/journal/european-urology/vol/79/suppl/S1 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Research Center for Evidence-based medicine, Iranian EBM Centre: A Joanna Briggs Institute (JBI) Center of Excellence, Tabriz University of Medical, Tabriz, Iran. |
| نویسنده | نفر چندم مقاله |
|---|---|
| هانیه صالحی پورمهر | اول |
| سکینه حاج ابراهیمی | دوم |
| جواد محمودی | سوم |
| سعید صدیق اعتقاد | چهارم |
| لیلا روشنگر | پنجم |
| رضا رهبرقاضی | ششم |
| نسرین ابوالحسن پور | هفتم |
| هادی مصطفایی | هشتم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Mesenchymal stem cells,spinal cord injury, neurogenic bladder |
| خلاصه مقاله | Introduction & objectives: Employment of mesenchymal stem cells (MSCs) therapy represents an emergent strategy in neuro-urology field. We aimed to investigate whether intra-detrusor injection of MSCs is a possible therapy for spinal cord injury (SCI) -induced neurogenic bladder (NGB). Materials & methods: Forty-two adult female Wistar rats were divided into 6 groups. Control group didn’t receive any intervention, rats in sham group underwent T9 laminectomy by a posterior midline approach without spinal cord damage; complete transection SCI (cSCI) and hemisection SCI (hSCI) rats underwent T9 transection and hemisection of the spinal cord, respectively and then received a single injection of normal saline into bladder wall 4 weeks post-injury in 6 areas of bladder muscle. Animals in cSCI + MSCs and hSCI + MSCs groups received 1×106/120µl injection of bone marrow MSCs (BM – MSCs) into bladder wall, 4 weeks post-injury. Four weeks post-transplantation animals sacrificed for sampling and molecular evaluations. Results: The number of apoptotic (TUNEL positive) cells was increased after SCI induction in bladder tissue (p<0.001). In contrast, transplantation of the BM-MSCs decreased it in hSCI-MSC group (p<0.05) (Figure 1). Immunohistochemistry imaging showed that the induction of hSCI and cSCI could increase the number of Ki-67 positive cells compared to the control and sham groups (Figure 2). We found that injection of BM-MSCs could increase the number of Ki-67 cells in hSCI compared to the control-matched groups. These data showed that BM-MSCs could possibly increase the cell proliferation rate in rats with hSCI (p=0.037), but not in cSCI (p=0.468). The existence of arterioles with α-SMA is a hallmark of mature vascularization. We found that microvascular density was increased in groups received MSCs compared to the control-matched groups. These data support a notion, that transplantation of rat BM-MSCs could restore urinary bladder function possibly by promoting angiogenesis and trans-differentiation into myocytes. Conclusion: Our results showed improvement of rat’s bladder function following MSCs transplantation into detrusor muscle of hemisection SCI-induced NGB via modulation of myogenesis, angiogenesis, trophic factors and apoptosis. Intra-detrusor wall BM-MSCs transplantation may be a novel therapeutic strategy for bladder dysfunction in SCI patients at the chronic phase suffering from NGB disorder. It seems that the results of this study help us to develop an alternative modality for alleviation of neuro-urology disorders. Neuro-regeneration in stem cell therapy to correct bladder dysfunction is a logical and promising strategy. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Certificate_EAU21.pdf | 1400/06/15 | 554795 | دانلود |
| EAU.pdf | 1400/06/16 | 305856 | دانلود |
