چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Background: Alongside many complications in understanding the etiology of Preeclampsia (PE), several determinants, such as the imbalanced proportion of anti-angiogenic/proangiogenic T-cell subsets, especially CD4+ (Th17/Treg), as well as alterations in the expression profle of related cytokines, miRNAs, and transcription factors might have been implicated in PE pathogenesis. Material and method: After sample collection and preparation, CD4+ cells were isolated from PE and non-PE pregnant woman and were cultured. Furthermore, analysis such as flow cytometry, real-time PCR, western blotting, and ELISA were performed to assess determinants related to PE manifestation, including sFlt-1, sEng, STAT-3, RORγt, SMAD-7, Foxp3, IL-17, IL-22, Ets-1, and miRNA-326. Results: Our results showed that the miRNA-326 expression level increased in CD4+ Cells and Th17 in PE patients which downregulated Ets-1 expression that acts as a negative control for Th17 development. Furthermore, we showed that the number and expression level of Th17 s and transcription factor RORγt escalated, respectively. While Treg and its related transcription factor (Foxp3) demonstrated a decrease. Flow cytometry analysis illustrated that the Th17/Treg ratio increased in PE. Additionally, we demonstrated that expression and concentration levels of cytokines (IL-17 and IL22) and anti-angiogenic molecules (sEng and sFlt-1) soared in isolated CD4+ cells from PE patients, which could be correlated with PE pathogenicity. Conclusion: In conclusion, we comprehensively evaluated immunological factors and molecules involved in PE manifestation. Interestingly, the CD4+ T-cell subset could be an extra source of antiangiogenic factors for the maintenance of this hypertension disorder. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمد علی ذوالفقاری | اول |
| رزا متولی خیاوی | دوم |
| محمدصادق سلطانی زنگبار | سوم |
| فروغ پرهیزکار | چهارم |
| لیلی عاقبتی | ششم |
| محمد نوری | هفتم |
| صنم دولتی | هشتم |
| مجید احمدی | نهم |
| حسین صمدی کفیل | دهم |
| فرهاد جدیدی نیارق | یازدهم |
| عطا محمودپور | سیزدهم |
| محمد سعید حجازی | چهاردهم |
| مهدی یوسفی | پانزدهم |
| جواد احمدیان هریس | دوازدهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| A new approach to the preeclampsia puzzle; MicroRNA-326 in CD4+.pdf | 1400/05/16 | 4631096 | دانلود |
