چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Gene Reports 24 (2021) 101274Available online 7 July 20212452-0144/© 2021 Elsevier Inc. All rights reserved.Restoration of miR-124 serves as a promising therapeutic approach in CRC by affecting CDK6 which is itself a prognostic and diagnostic factor Morteza Akbaria,b,c, Ali Adilid, Afsaneh Farajib, Abbas Pakdele, Ramin Aslaminabadb, Davoud Nasrabadib, Shahram Sadeghvandf, Hossein Saeedib,g, Mina Tahavorig,h, Aliakbar Shabania,c,*, Behzad Baradaranb,** aDepartment of Biotechnology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran bImmunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran cSemnan Biotechnology Research Center, Semnan University of Medical Sciences, Semnan, Iran dDepartment of Oncology, Tabriz University of Medical Sciences, Tabriz, Iran eDepartment of Biochemistry, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran fChildren Hospital, Tabriz University of Medical Sciences, Tabriz, Iran gStudent Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran hDepartment of Pharmaceutics, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran ARTICLE INFO Keywords: Colorectal Cancer 5-FU Oxaliplatin miR-124 CDK6 Prognosis Diagnosis Treatment ABSTRACT Background: Colorectal cancer is one of the most common cancers in the world. 5 Fluorouracil and Oxaliplatin are two common chemotherapeutic agents are used in patients with CRC. miR-124 and CDK6 are two notable genes that have been shown to play important roles in various cancers, including colorectal cancer. Objective: In this study, we investigated the expression of miR-124 and CDK6 in patients with colorectal cancer. Then, the effect of miR-124 and chemotherapeutic drugs on CDK6 expression and cell viability was assessed in the SW480 CRC cell line. Also, the relationship between miR-124 and CDK6 with the clinicopathological features of the patients was evaluated. Materials and methods: Materials and Methods: Colorectal cancer and its corresponding non-tumor tissues were collected from 50 patients the relative expression of miR-124 and CDK6 was assessed by Real-Time PCR. The relationship between the pathologic features of the patients and the target genes was also evaluated. In addition, the relative expression of CDK6 in response to miR-124 and its combination with 5-FU and Oxaliplatin was evaluated in the SW480 cell line by Real-Time PCR and Western blot analysis. Besides, the effect of miR-124 and chemotherapeutic drugs on cell viability was determined by MTT assay. Results: miR-124 was down-regulated in CRC patients while CDK6 was up-regulated in CRC tissues compared with adjacent non-tumor healthy controls. In addition, CDK6 was significantly correlated with pathologic fea-tures of patients including stage and differentiation. Moreover, CDK6 expression and cell viability were atten-uated in response to miR-124, 5-FU, and Oxaliplatin in the SW480 cell line; however, the effect was prominent when miR-124 was transfected with chemotherapeutic drugs. Conclusion: The results of our study suggest that miR-124 reinforcement may act as a promising therapeutic approach for CRC by affecting CDK6. Nevertheless, it may act as a supreme therapeutic method when combines with chemotherapy drugs. Besides, CDK6 may be used as a diagnostic marker to determine the clinical outcome of patients with colorectal cancer. |
| نویسنده | نفر چندم مقاله |
|---|---|
| علی عدیلی | دوم |
| افسانه فرجی | سوم |
| شهرام صادق وند | هفتم |
| حسین سعیدی | هشتم |
| بهزاد برادران | یازدهم |
| رامین اصل امین آباد | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 541-Restoration of miR-124 serves as a promising therapeutic approach in CRC by affecting CDK6 which is itself a prognostic and diagnostic factor.pdf | 1400/05/12 | 807991 | دانلود |
