| The purpose of this study was to investigate the effects of injection of CO2 / N2
gases at different ratios and gas treatment duration on physicochemical and
microbiological properties of raw milk using a combined design. In this
research acidity, pH,freezing point, total count, total yeast and mold count
andtotal coliform count were performedon 15 raw milk samples between 9 and
15 minutes after injections. Statistical analysis showed that the combined
treatment of nitrogen and carbon dioxide had significant effect on the acidity
and pH of raw milk (p˂0.05), so that with increasing the amount of carbon
dioxide to 100%, the pH decreased and acidity increased. The combined effect
of both gases on the freezing point of raw milk was significant (p˂0.05).
Increasing the treatment time of raw milk from 9 to 15 minutes had significant
effect (p˂0.05) on reducing the microbial load, but no significant reduction
was observed due to the combination of gases. According to ANOVA results,
in 100% nitrogen and duration of 15 minutes, the best results in terms of
microbiology and chemistry were obtained. The effect of milk treated with a
mixture of CO2 and N2 gases on yogurt properties, as a food model, has also
been investigated. Yogurt samples were investigated for chemical, microbial,
and organoleptic properties during 22 days storage time. The results showed
that the raw milk treated with gas mixture did not have an adverse significant
effect on the sensory and physicochemical properties of yogurt (p> 0.05).
However, yogurt acidity, pH and synthesis were significantly affected during
storage time (p˂0.05). Therefore, the application of raw milk treatment with
100% nitrogen gas for 15 minutes can be considered as a bacteriostatic agent
in the preservation of raw milk in industrial scale. |