چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| In the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles (AuNPs) modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01 fg mL-1 to 1 pg mL-1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL-1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia (CLL) suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability. |
| نویسنده | نفر چندم مقاله |
|---|---|
| روزیتا ابوالحسن | اول |
| بلال خلیل زاده | دوم |
| فروغ چاکری خیاوی | پنجم |
| فرزانه قربانی | ششم |
| رامین پوراکبری | هفتم |
| امین کامرانی | هشتم |
| محمدرضا رشیدی شاهگلی | یازدهم |
| مهدی یوسفی | دوازدهم |
| لیلی عاقبتی | سیزدهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abolhasan_et_al-2021-Scientific_Reports.pdf | 1400/05/03 | 3224348 | دانلود |
