چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Mesenchymal stem cells (MSCs) as an undifferentiated cells are specially considered in cell-based cancer therapy due to unique features such as multi-potency, pluripotency, and self-renewal. A multitude of cytokines secreted from MSCs are known to give such multifunctional attributes, but details of their role are yet to be unknown. In the present study, MSCs were cultured, characterized and co-cultured with Molt-4 cells as acute lymphoblastic leukemia cell line in a trans-well plate. Then, cultured Molt-4 alone and Molt-4 co-cultured with MSCs (10:1) were collected on day 7 and subjected to Real time-PCR and western blotting for gene and protein expression assessment, respectively. Ki-67/Caspase-3 assay as well as telomere length measurement, were investigated by flow cytometry and Real time-PCR, respectively. The results showed that MSCs caused significant decrease in telomere length as well as hTERT gene expression of Molt-4 cells. Also, it was pointed that gene and protein expression of BAD and P53 were significantly increased. In the following, the flow cytometry analysis indicated the decrease and increase of the Ki and caspaspase-3 expression, respectively. It was concluded that MSCs co-cultured with Molt-4 cells could be involved in the promotion of Molt-4 cell apoptosis via caspase-3, BAD, and P53 expression. In addition, the decrease of telomere length is another effect of MSCs on Molt-4 leukemic cells. |
| نویسنده | نفر چندم مقاله |
|---|---|
| حمیدرضا حیدری سورشجانی | اول |
| عزت اله فتحی | دوم |
| سهیلا منتظرصاحب | سوم |
| ایوب ممندی | چهارم |
| راحله فرحزادی | پنجم |
| سید سوران زلاوی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| ijmcmed-v10n2p113-en.pdf | 1400/06/12 | 639115 | دانلود |
