چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Finding new strategies for heart failure treatment based on using stem cells has attracted a great deal of attention. Meanwhile, nanotechnology-based approaches to regenerative medicine hypothesize a possible strategy between stem cells and nanotechnology in the treatment of disease. This study aims to investigate the in vitro effect of silver nanoparticles (Ag-NPs) on cardiomyogenic differentiation of bone marrow-derived mesenchymal stem cells (BM-MSCs) through detection of cardiac markers. For this purpose, MSCs were isolated from bone marrow resident and differentiated to the cardiac cells using a dedicated medium with Ag-NPs. Also, the cardiomyogenic differentiation of BM-MSCs was confirmed using immunocytochemistry. Then, the cells were subjected to real-time PCR and western blotting assay for absolute telomere length (TL) measurement, gene and protein assessment, respectively. It was found that 2.5 µg/ml Ag-NPs caused elongation of the TL and altered VEGF, C-TnI, VWF, SMA, GATA-4, TERT, and Cyclin D protein and gene expression in the cardiomyogenic differentiated BM-MSCs. Also, there was a significant increase in the protein and gene expression of Wnt3 and β-catenin as a main component of pathways. We concluded that Ag-NPs could change the in vitro cardiac markers’ expression of BM-MSCs via Wnt3/β-catenin signaling pathway. |
| نویسنده | نفر چندم مقاله |
|---|---|
| خسرو ادیب کیا | اول |
| علی احسانی | دوم |
| اسماء جدائی | سوم |
| راحله فرحزادی | پنجم |
| محمد برزگر جلالی | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Beilstein J Nanotschnology.pdf | 1400/05/12 | 4389562 | دانلود |
