An innovative flexible and portable DNA based biodevice towards sensitive identification of Haemophilus influenzae bacterial genome: A new platform for the rapid and low cost recognition of pathogenic bacteria using point of care (POC) analysis
An innovative flexible and portable DNA based biodevice towards sensitive identification of Haemophilus influenzae bacterial genome: A new platform for the rapid and low cost recognition of pathogenic bacteria using point of care (POC) analysis
نویسندگان: آرزو سعادتی , هومن خلفازاد کردشت , مریم احسانی , محمد حسن زاده محله
کلمات کلیدی: Haemophilus influenzae, Biomedical electroanalysis, DNA biosensor, Nanomaterials, Biodevice, Point of care
نشریه: 24084 , 2021 , 169 , 2021
| نویسنده ثبت کننده مقاله |
محمد حسن زاده محله |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
مرکز تحقیقات آنالیز دارویی |
| کد مقاله |
76360 |
| عنوان فارسی مقاله |
An innovative flexible and portable DNA based biodevice towards sensitive identification of Haemophilus influenzae bacterial genome: A new platform for the rapid and low cost recognition of pathogenic bacteria using point of care (POC) analysis |
| عنوان لاتین مقاله |
An innovative flexible and portable DNA based biodevice towards sensitive identification of Haemophilus influenzae bacterial genome: A new platform for the rapid and low cost recognition of pathogenic bacteria using point of care (POC) analysis |
| ناشر |
6 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
بلی |
| عنوان نشریه (خارج از لیست فوق) |
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| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
|
| Nowadays, different respiratory infections of Haemophilus Influenzae (H. influenzae) such as bronchitis, pneumonia, acute otitis media and sinusitis reveals the high demand for developing efficient, rapid and low-cost sensing platforms. In this concept, pDNA and cDNA were exploited in electrochemical biosensors for monitoring H. influenzae. Accordingly, the Ag ink and Au@Ag core–shell/GQDs nano-ink were synthesized and drawn on the surface of photographic paper for designing a three-electrode paper-based biodevice. The selectivity of the designed platform was evaluated by three mismatched. Under the optimal experimental conditions, due to the high affinity of cDNA toward pDNA, the fabricated electrochemical bioassay provided good selectivity and sensitivity for the monitoring of H. influenzae. The developed flexible sensor could detect cDNA with linear range and LLOQ (lower limit of quantitation) 100 ZM to 0.1 pM and 100 ZM, respectively. The main advantages of this paper-based DNA bio-electrochemical sensor include acceptable sensitivity, selectivity, easy and fast operation to clinical analysis and biomedicine. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| 280-Article.pdf | 1400/04/25 | 8343763 | دانلود |
