Spectrofluorimetric determination of indoxyl sulfate in human plasma after salting‑out assisted liquid–liquid extraction
Spectrofluorimetric determination of indoxyl sulfate in human plasma after salting‑out assisted liquid–liquid extraction
نویسندگان: علی شایان فر , ابوالقاسم جویبان , فاطمه نوروزی , افشین قره خانی
کلمات کلیدی: Chronic kidney disease patients · Indoxyl sulfate · Salting-out assisted liquid–liquid extraction
نشریه: 6564 , 2021 , 75 , 2021
| نویسنده ثبت کننده مقاله |
علی شایان فر |
| مرحله جاری مقاله |
تایید نهایی |
| دانشکده/مرکز مربوطه |
مرکز تحقیقات آنالیز دارویی |
| کد مقاله |
76309 |
| عنوان فارسی مقاله |
Spectrofluorimetric determination of indoxyl sulfate in human plasma after salting‑out assisted liquid–liquid extraction |
| عنوان لاتین مقاله |
Spectrofluorimetric determination of indoxyl sulfate in human plasma after salting‑out assisted liquid–liquid extraction |
| ناشر |
4 |
| آیا مقاله از طرح تحقیقاتی و یا منتورشیپ استخراج شده است؟ |
خیر |
| عنوان نشریه (خارج از لیست فوق) |
|
| نوع مقاله |
Original Article |
| نحوه ایندکس شدن مقاله |
ایندکس شده سطح یک – ISI - Web of Science |
| آدرس لینک مقاله/ همایش در شبکه اینترنت |
|
| A simple analytical method was developed to quantify indoxyl sulfate (InS) in plasma using a salting-out assisted liquid–
liquid extraction and spectrofluorimetry. Plasma samples were deproteinized by acetonitrile. Then, sodium chloride solution
was added to separate acetonitrile and aqueous phase. The fluorescence intensity of InS which extracted to acetonitrile
phase was determined by spectrofluorimetry. The method of presentation has a linear range between 2.5 and 40 μg/ml with
a coefficient of determination (R2) of 0.995. Accuracy (% error) and precision (relative standard deviation) were 15% at
nominal concentration and less than 15% at all levels, respectively. This established analytical method, which is sensitive
and reproducible, could be applied for the quantification of InS in real plasma samples. |
| نام فایل |
تاریخ درج فایل |
اندازه فایل |
دانلود |
| Norouzi et al-Chemical papers 2021.pdf | 1400/04/19 | 676675 | دانلود |