چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| It has been shown that near all organs, especially the cardiovascular system, are afected by bacterial lipopolysaccharide via the activation of Toll-like receptor signaling pathways. Here, we tried to fnd the blunting efect of bacterial lipase on lipopolysaccharide (LPS)-induced cardiac tissue toxicity in chicken embryos. 7-day fertilized chicken eggs were divided randomly into diferent groups as follows; Control, Normal Saline, LPS (0.1, 0.5 and 1 mg/kbw), and LPS (0.1, 0.5 and 1 mg/kbw) plus 5 mg/ml Lipase. On day 17, the hearts were sampled. The expression of genes such as GATA4, NKX2.5, EGFR, TRIF, and NF-ƙB was monitored using real-time PCR analysis. Using western blotting, we measured NF-ƙB protein level. Total antioxidant capacity, glutathione peroxidase, and Catalase activity were also studied. Microvascular density and anterior wall thickness were monitored in histological samples using H&E staining. High dose of LPS (1 mg/kbw) increased the expression of TRIF but not NF-ƙB compared to the control group (p<0.05). We found a statistically signifcant reduction in groups that received LPS+Lipase compared to the control and LPS groups (p<0.05). Western blotting revealed that the injection of Lipase could reduce LPS-induced NF-ƙB compared to the control group (p<0.05). The expression of GATA4, NKx2.5, and EGFR was not altered in the LPS group, while the simultaneous application of LPS and Lipase signifcantly reduced GATA4, NKx2.5, and EGFR levels below the control (p<0.05). We found non-signifcant diferences in glutathione peroxidase, and Catalase activity in all groups (p>0.05), while total antioxidant capacity was increased in groups that received LPS+Lipase. Anterior wall thickness was diminished in LPS groups and the use of both lipase and LPS returned near-to-control values (p<0.05). Despite a slight increase in microvascular density, we found statistically non-signifcant diferences in all groups (p>0.05). Bacterial lipase reduces detrimental efects of LPS on chicken embryo heart induced via Toll-like receptor signaling pathway. |
| نویسنده | نفر چندم مقاله |
|---|---|
| فاطمه سکوتی | سوم |
| شاهین احمدیان | چهارم |
| مهرداد فرهادی | ششم |
| فریبا یحیوی | هفتم |
| بهنام هاشمی | هشتم |
| رضا رهبرقاضی | نهم |
| مهدی مهدی پور | دهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Article.pdf | 1400/02/07 | 1936338 | دانلود |
| 728.jpeg | 1400/02/11 | 58316 | دانلود |
