کاربرد تکنیک های تحلیلی با وضوح بالا در مطالعات تنفسی
Application of high-resolution analytical techniques in the breathomics studies
مجریان: جعفر سلیمانی
خلاصه روش اجرا: We gathered about 150 detailed scientific papers and 12 review articles published online from 2000 to 2023 in case of breath analysis studies. The number of studies included was 82. The search terms included, “metabolomics”, “breathomics”, “volatile organic compounds”, “high-resolution AND breath analysis,”, “mass spectrometry AND breath analysis”, “Liquid chromatography AND breath analysis”, “NMR AND breath analysis”, and “gas chromatography AND breath analysis” in the title and keywords. The search language was English. A wide range of relevant reports were searched using Science Direct, PubMed/Medline, and Scopus search engines.Techniques combined with mass spectroscopy are expected to provide more detailed information. However, fluorescent and absorbance-based techniques have significant weaknesses in brethomics. In presenting the methods for measuring analytes in exhaled breath samples, we will analyze their advantages and disadvantages. Additionally, the results obtained will be compared with those from other methods.
اطلاعات کلی طرح 
| مرحله جاری طرح | خاتمه قرارداد و اجرا |
| کد طرح | 75490 |
| عنوان فارسی طرح | کاربرد تکنیک های تحلیلی با وضوح بالا در مطالعات تنفسی |
| عنوان لاتین طرح | Application of high-resolution analytical techniques in the breathomics studies |
| نوع طرح | گرنت پژوهشی |
| اولویت طرح | پیشگیری، اپیدمیولوژی و درمان و بازتوانی بیماریهای التهابی راههای هوایی (آسم و COPD) |
| نوع مطالعه | مطالعات مروری |
| تحقیق در نظام سلامت | بلی |
| آیا طرح پایاننامه دانشجویی است؟ | خير |
| مقطع پایان نامه | |
| مدت اجرا - ماه | 12 |
| نوآوری و ضرورت انجام تحقیق | Given the promising results reported in the literature on metabolomics for early disease diagnosis, the review will prepare with aims to discuss advances of all high-resolution techniques in exhaled breath analysis and provide a comprehensive and up-to-date review of them. Additionally, the review will offer a summary of the benefits and drawbacks of the reported breath analysis techniques as best practices for data collection and provides insight into potential future research. |
| اهداف اختصاصی | Provide a summary of the impact of the high resolution techniques for the reported breath analysis techniques |
| چکیده انگلیسی طرح | Human exhaled breath has gained popularity in recent decades due to its accessible and easily obtainable nature, making it a promising matrix for metabolomics studies. The equilibrium of the dissolved volatile organic compounds (VOCs) between blood and lung gases enables their monitoring in the gas phase. Therefore, analyzing VOCs in alveolar air can serve as an appropriate indicator of the pulmonary blood concentration of these compounds. Currently, there is compelling evidence indicating that the VOC profile can provide valuable insights into individual metabolic processes. In addition to being widely available and noninvasive, breath analysis is also utilized for molecular fingerprinting, offering the potential for the development of point-of-care instruments. However, the translation of findings from single-center studies to large-scale and multicenter levels is now necessary. To date, international guidelines have approved a limited number of breath analysis tests for disease diagnosis, but none of them are capable of serving as stand-alone diagnostic tests. This review aims to discuss the advancements in exhaled breath analysis studies, focusing on the promising profiles reported in the literature regarding metabolomics for early disease diagnosis. While presenting the methods used to measure analytes in exhaled breath samples, their advantages and disadvantages will be analyzed. The obtained results will also be compared and analyzed with those from other methods. |
| کلمات کلیدی | metabolomics-the detection, characterization, and quantification of the endogenous low-molecular weight metabolites (>650 Da) in a biological system including small acids, alcohols, carbohydrates, amino acids, fatty acids, lipids, sterols, catecholamines, drugs, and toxins volatile organic compounds (VOCs)-VOCs are produced within the bodily metabolic processes released to the blood circulation, and exhaled in breath via alveolar membrane breathomics-is a new branch of a multidisciplinary studies with the core of metabolomics exhaled breath vapor (EBV) -EBV samples are directly collected by breathing into specific bags and consist of N2, O2, CO2, water vapor, and a mixture of VOCs exhaled breath condensate (EBC)-EBC is mainly comprised of water, soluble gases, and trace concentration of metabolites of the extracellular fluids. Thus, in comparison to EBV, EBC has a more complex composition |
| ذینفعان نتایج طرح | medical affairs |
اطلاعات مجری و همکاران
| نام و نامخانوادگی | سمت در طرح |
|---|---|
| زهرا گل صنم لو | همکار اصلی |
| جعفر سلیمانی | مجری اول (اصلی-هیات علمی) |
| ابوالقاسم جویبان | همکار اصلی |
اطلاعات تفضیلی
| حوزه خبر | خبر |
|---|---|
| رسانه ها و مردم | عنوان خبر متن خبر |
| متخصصان و پژوهشگران | عنوان خبر کاربرد تکنیک های تحلیلی با وضوح بالا (high-resolution) در مطالعات تنفسی بررسی شد.متن خبر در این مطالعه مروری، تاثیر تکنیک های تحلیلی با وضوح بالا (high-resolution) در مطالعات تنفسی مورد مطالعه قرار گرفت. در مطالعات تنفسی، استفاده از تکنیکهای تحلیلی با وضوح بالا (high-resolution) مانند کروماتوگرافی گازی طیفسنجی جرمی (GC-MS) و طیفسنجی لیزری امکان شناسایی دقیق ترکیبات فرار در بازدم را فراهم میکند. این روشها با دقت بالا، حتی اجزای کمغلظت مانند متابولیتهای بیماریزا یا بیومارکرهای تنفسی را تشخیص میدهند. بهویژه در تحقیقات مربوط به آسم، COPD و عفونتهای ریوی، این فناوریها به درک بهتر مکانیسمهای بیماری و توسعه روشهای تشخیص غیرتهاجمی کمک شایانی کردهاند. همچنین، ترکیب این تکنیکها با هوش مصنوعی، تحلیل الگوهای پیچیده تنفسی را تسهیل نموده است. این مطالعه، با بررسی نظامند هر یک از دستگاههای تحلیلی با وضوح بالا معایب و مزایا و همچنین آینده هریک از تکنیک ها را در مطالعات تنفسی تحلیل کرده است. |
| سیاستگذاران درمانی | عنوان خبر متن خبر |
| سیاستگذاران پژوهشی | عنوان خبر متن خبر |
| لینک (URL) مقاله انگلیسی مرتبط منتشر شده 1 |
