چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | امیر مهدی زاده حقیقی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز تحقیقات غدد درون ریز |
| کد مقاله | 75417 |
| عنوان فارسی مقاله | The effect of diabetic serum free fatty acids on the stemness markers of adipose tissuederived mesenchymal stem cells |
| عنوان لاتین مقاله | The effect of diabetic serum free fatty acids on the stemness markers of adipose tissuederived mesenchymal stem cells |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | 16th National Congress of Biochemistry & 7th International Congress of Biochemistry & Molecular Biology |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1399 |
| سال انتشار/ارائه میلادی | 2020 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1399/08/09 الی 1399/08/11 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://icbmb.ir/index.php?&slct_pg_id=10&sid=1&slc_lang=en |
| آدرس علمی (Affiliation) نویسنده متقاضی | Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| پریسا فیاض پور | اول |
| اشکان کلانتری چروده | دوم |
| سید وحید حسینی | سوم |
| میترا نیافر | چهارم |
| وحیده صدرا | پنجم |
| امیر مهدی زاده حقیقی | ششم |
| مسعود دارابی امین | هفتم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | free fatty acids, mesenchymal stem cells, diabetes, gas-liquid chromatography |
| خلاصه مقاله | Background Diabetes mellitus (DM) is caused by deficient insulin secretion or insulin resistance leading to dyslipidemia. Human adipose tissue-derived mesenchymal stem cells (AdMSCs) are crucial for the regeneration process of adult tissues. Since regenerative capacity is impaired in DM, this study aimed to evaluate the effect of diabetic serum free fatty acids (FFAs) on stemness of AdMSCs in vitro. Methods AdMSCs were retrieved from abdominal adipose tissue samples. Pooled diabetic and nondiabetic serums were then prepared from 7 and 8 women in menopausal ages (up to 65 year), respectively. AdMSCs were treated with diabetic serum (DS), non-diabetic serum (NDS), diabetic FFAenriched serum (DFS) and non-diabetic FFA-enriched serum (NDFS) for 48 hours. FFA composition was analyzed using gas-liquid chromatography. The expression of stemness markers CD49e and CD90 were also analyzed by quantitative PCR. Cell proliferation was evaluated by BrdU assay. The study was approved by the local ethics committee (No. IR.TBZMED.REC.1398.659). Results A significantly higher oleate level was observed in DS (1.4-fold, p<0.05) compared to NDS. Compared to NDS, DS significantly decreased CD49e expression (0.4±0.24 versus 1.05±0.43-fold change, p<0.05, respectively). However, no significant differences were observed in CD90 expression between the groups. Compared to NDFS, DFS significantly decreased the CD49e and CD90 expression (4.14±0.08 versus 6.2±0.46-fold change, p<0.05, and 1.34±0.17 versus 2.74±0.5, p<0.05, respectively). Additionally, DFS significantly decreased AdMSCs proliferation rate in contrast to NDFS (68.0%±1.0 versus 105.36%±0.65, p<0.05). Conclusion The negative effect of DS on AdMSCs stemness maintenance may be due to the higher oleate level. Therefore, controlling this fatty acid level in DM can be considered as a strategy for improving regeneration capacity in diabetic complications such as ulcers and delayed wound healing. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Conference proceeding-The effect of diabetic serum free fatty acids on the stemness markers.pdf | 1399/12/26 | 185874 | دانلود |
| govahi.pdf | 1400/01/21 | 366815 | دانلود |
