چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مسعود دارابی امین |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | مرکز سلولهای بنیادی |
| کد مقاله | 75416 |
| عنوان فارسی مقاله | Immediate stearoyl-CoA desaturase-1 activity is essential for endodermal differentiation of human induced pluripotent stem cells |
| عنوان لاتین مقاله | Immediate stearoyl-CoA desaturase-1 activity is essential for endodermal differentiation of human induced pluripotent stem cells |
| نوع ارائه | سخنرانی |
| عنوان کنگره / همایش | 16th National Congress of Biochemistry & 7th International Congress of Biochemistry & Molecular Biology |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1399 |
| سال انتشار/ارائه میلادی | 2020 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1399/08/09 الی 1399/08/11 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://icbmb.ir/index.php?&slct_pg_id=10&sid=1&slc_lang=en |
| آدرس علمی (Affiliation) نویسنده متقاضی | Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| سید وحید حسینی | اول |
| اشکان کلانتری چروده | دوم |
| پریسا فیاض پور | چهارم |
| مسعود دارابی امین | ششم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Human iPS cells, endoderm, stearoyl-CoA desaturase, stem cells |
| خلاصه مقاله | Background: Stearoyl-CoA desaturase 1 (SCD1) is required for de novo synthesis of fatty acid and is associated with protein posttranslational modification through fatty acid acylation process. In this study, we evaluated whether the dynamic of SCD1 activity is important in differentiation of human induced pluripotent stem cells (hiPSCs) to endoderm lineage using a chemical inhibitor, biochemical methods and immunostaining. Methods: The hiPSCs were cultured in an endoderm inducing medium containing activin A and low defined fetal bovine serum in the presence of an SCD1 inhibitor at different time points. The yield of three germs layers’ endoderm, mesoderm and ectoderm was assessed by measuring the surface markers CXCR4, KDR, SSEA-3 and NCAM using flow cytometry. The expression of endoderm specific markers Sox17 and CXCR4 and pluripotency markers Sox2 and Oct4 was assessed by means of Western blotting. Total protein acylation was evaluated using click chemistry reaction and the cell cycle analysis was performed using flow cytometry. The study was approved by the local ethics committee (No. IR.TBZMED.REC.1395.680). Results: The population of cells with endodermic features decreased at the end of differentiation when SCD1 was inhibited at the first day and effectively rescued by oleate supplementation. Moreover, SCD1 inhibition at day 0 preserved hiPSCs stem cell properties without a shift toward mesoderm or ectoderm. Only, treatment of cells with SCD1 inhibitor at first day decreased the intensity of fluorescent in the click chemistry. Treatment at two subsequent days of endoderm induction induced no significant effect on endoderm specific markers and fluorescent intensity. Reproducible results were also obtained with a human embryonic stem cell line. Conclusion: SCD1 activity is required for the initiation and commitment of endodermal differentiation from hiPSCs. The requirement for SCD1 activity in endodermal differentiation of ESCs may be eminent in disorders of endoderm-derived organs and dysregulated metabolism. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract-Immediate stearoyl-CoA desaturase-1 activity is essential.pdf | 1399/12/26 | 263554 | دانلود |
| oral certificate presentation-vh.jpg | 1399/12/26 | 87021 | دانلود |
