چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| One of the most important natural extracellular constituents is hyaluronic acid (HA) with the potential to develop a highly organized microenvironment. In the present study, we enriched HA hydrogel with tenascin-C (TN-C) and examined the viability and survival of mouse neural stem cells (NSCs) using different biological assays. Following NSCs isolation and expansion, their phenotype was identified using flow cytometry analysis. Cell survival was measured using MTT assay and DAPI staining after exposure to various concentrations of 50, 100, 200, and 400 nM TN-C. Using acridine orange/ethidium bromide staining, we measured the number of live and necrotic cells after exposure to the combination of HA and TN-C. MTT assay revealed the highest NSCs viability rate in the group exposed to 100 nM TN-C compared to other groups and a combination of 1% HA +100 nM TN-C increased the viability of NSCs compared to the HA group after 24 hours. Electron scanning microscopy revealed the higher attachment of these cells to the HA+ 100 nM TN-C substrate relative to the HA substrate. Epifluorescence imaging and DAPI staining of loaded cells on HA + 100 nM TN-C substrate increased significantly the number of NSCs per field over 72 hours compared to the HA group (p<0.05). Live and dead assay revealed that the number of live NSCs significantly increased in the HA + 100 TN-C group relative to HA and control groups. The enrichment of HA the substrate with TN-C promoted NSCs viability and survival and could be applied in neural tissue engineering approaches and regenerative medicine. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مریم شاهی | اول |
| داریوش محمدنژاد | دوم |
| محمد کریمی پور | سوم |
| رضا رهبرقاضی | چهارم |
| علی عابدالهی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Evaluation of the Effect of Hyaluronic Acid Based Biomaterial Enriched With Tenascin C on the Behavior of the Neural Stem Cells.pdf | 1400/02/04 | 2298695 | دانلود |
| aabed.png | 1400/02/06 | 196937 | دانلود |
