چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Human 2-Arachidonoylglycerol (2-AG) is an agonist of endocannabinoid system and acts as an important modulator of many physiological processes such as emotional state and pain sensation. Identification and quantification of 2-AG is vital for medical and pathological processes. There's no report for the measurement of 2-AG in human biofluids using modern methods such as biosensors. This study reports an ultra-sensitive and selective immunosensor to determination of endocannabinoids 2-AG in human plasma samples. In this study, gold nano-flowers (AuNFs) were synthesized and conjugated with specific biotinylated antibody of 2-AG. Bioconjugated composite (bioreceptor with AuNFs) was immobilized on the surface on gold electrode and used for the monitoring of antigen (Target molecules) based on immunoreaction process. Also, constructed interface was characterized by field emission scanning electron micro-scope (FE-SEM), dynamic Light Scattering (DLS), Transmission electron microscopy (TEM) and zeta potential methods. Using proposed immuno-platform, 2-AG was determined in two dynamic ranges of 0.00024-0.0078 ng/L and 2-16 ng/L with lower limit of quantitation (LLOQ) of 0.00024 ng/L. These results recommended that our immunosensor might be appropriate for an early diagnosis of 2-AG towards screening of immunomodulatory and neuroprotection. |
| نویسنده | نفر چندم مقاله |
|---|---|
| احمد موبدکرکج | اول |
| علی احمدعلی پور | سوم |
| محمد حسن زاده محله | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 255.pdf | 1399/11/09 | 1101267 | دانلود |
| document.pdf | 1399/11/19 | 218675 | دانلود |
