چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| tAcetic acid-functionalized magnetic nanoparticles modified by (3-amino-propyl)-tri-ethoxy silane wassynthesized and used as a new solid-phases adsorbent. Infrared spectroscopy (FT-IR), scanning electronmicroscopy (SEM), x-ray diffraction (XRD), energy-dispersive x-ray spectroscopy (EDX), vibrating samplemagnetometer (VSM) and Electrophoretic Light Scattering (ELS) were used to characterize the modifiednanoparticles. The molecular interaction between letrozole and nanoparticles (NPs) was studied usingdensity functional theory (DFT) calculations. The developed nanoparticles were applied for dispersivesolid-phase extraction of letrozole (an anticancer drug) from human plasma. Extracted letrozole wasquantified using an isocratic HPLC/FL method. The extraction efficiency was optimized using one exper-iment at a time optimization method based on the adsorbent quantity, sample pH, adsorption time,desorption time, and elution solvent type/volume. The analysis method was fully validated according tothe FDA guideline for bioanalytical method validation. The linear quantification range was 0.01−1 g/mLand the lower limit of quantification (LLOQ) was 0.01 g/mL. Plasma samples of 6 patients were analyzedand the measured letrozole concentrations range was 0.04−0.31 g/mL. The newly synthesized magneticnanoparticles were used successfully for the extraction of letrozole from spiked and clinical plasma sam-ples. The developed method is a precise and simple method that is suitable for pharmacokinetic studiesand clinical applications. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مینا شبان | اول |
| صبا غفاری | دوم |
| جلال حنائی | سوم |
| سمیه سلطانی | پنجم |
| آیدا کاربخش زاده | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 1717efd30ba2337a0417649aebe21818.pdf | 1399/10/07 | 2270178 | دانلود |
