چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| الهام احمدیان | اول |
| سیمین شریفی | دوم |
| سولماز ملکی دیزج | سوم |
| محمد سمیعی | چهارم |
| عزیز افتخاری | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | calciteriol , regeneration, differentiation, dental pulp stem cells |
| خلاصه مقاله | Calcitriol is the active form of vitamin D, normally made in the kidney. A synthetic formula is utilized to treat kidney disease with low blood calcium, hyperparathyroidism due to kidney disease, low blood calcium due to hypoparathyroidism, osteoporosis, osteomalacia, and familial hypophosphatemia. Stem cell differentiation plays an active role in the pathogenesis of the aforementioned diseases. The evidence report that calciteriol might stimulate bone remodeling through an prohibition or induction of osteocyte differentiation. MSCs are one of the main cell types implemented in regenerative medicine. DPSCs, an substitute source for MSCs, could develop a means for cell regenerative medicine and tissue engineering to be utilized in different kinds of bone defect induced by a congenital malformation, tumor, trauma, and age-related osteoporosis. Thus, in this study, the effect of calciteriol on the osteogenic differentiation of DPSCs are examined. In this study, for assessment of calciteriol effect on viability and proliferation of DPSCs was used from MTT assay Human DPSCs were induced with osteogenic differentiation medium: α-MEM medium supplemented with 10% FBS, 100 IU/ml penicillin, 100 μg/ml streptomycin, 10 nM dexamethasone, 0.2 mM sodium L-ascorbyl-2-phosphate, and 10 mM β-glycerol phosphate. Differentiation medium was changed every third day. Calciteriol was added in the differentiation media throughout the differentiation processes until harvest for assays. The gene expression level and activity of ALP was determined at day 7. The specific primers were used including: ALP, forward: 5′-GACCCTTGACCCCCACAAT-3′, reverse: 5′-GCTCGTACTGCATGTCCCCT-3 and GAPDH, forward: 5′-AGCCACATCGCTCAGACAC-3′, reverse: 5′-GCCCAATACGACCAAATCC-3′. The results were normalized by the expression of GAPDH. For Alkaline phosphatase activity assay, After 7 days of treatment, the cells grown under osteogenic media were harvested and resuspended in 250 μl of culture supernatants, followed by cell breaking with an ultrasound breaker. After centrifugation, the ALP activities in the cell supernatants were quantified by an ALP detection kit and a spectrophotometer at a wavelength of 520 nm. Each value was normalized to the protein concentration. We examined the DPSCs viability after treatment with calciteriol by MTT proliferation assay. (Figure 1). After 48,72 h and 7 days of incubation, cell viability was augmented meaningfully in the calciteriol low‐concentrations. The results of ALP activity test on DPSCs treated with caliteriol for 7 days showed a significant increase in ALP activity compared to the control group (p <0.05) (Figure 2).The results of ALP gene expression on DPSCs exposed to calciteriol, for 7 days, showed a significant increase in ALP gene expression compared to the control group. (p <0.05) (Figure 3). Owed to the positive effects of calciteriol on ALP activity and gene expression in DPSCs, it may be an opportunity in bone and dental tissues engineering. |
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