چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| وحیده طرح ریز | چهارم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | MicroRNAs (miRNA) are crucial regulators of a wide range of cellular processes and have been identified as promising various diseases biomarkers such as cancer due to the presence in serum. MicroRNA, recognized as mir181a, is involved in the mechanisms of immunity and also in many different cancers such as chronic lymphocytic leukemia, neuroblastoma, breast cancer and glioma. In the current research , the electrochemical DNA biosensor is presented in reference to glassy carbon electrode (GCE) for immobilization and detection of short microRNA-181a sequences with methylene blue (MB) as electroactive label within conducting polymer of polypyrrole (PPy). Electropolymerization of PPy at the surface of GCE was achieved employing cyclic voltammetry (CV) method. The polymerization process and surface properties of free PPy threated with microRNA-181a were confirmed by FTIR spectrophotometric and AFM techniques. Any hybridization of microRNA-181a as a probe at the surface of GCE with complementary sequences was accompanying MB signal variations. Hence, the states of ssDNA (microRNA-181a) and dsDNA (microRNA-181a-complementary strand) were monitored based on the MB signal as electroactive indicator. Results indicated that the modified GC electrode with PPy is capable in detecting a cancer biomarker of microRNA-181a at the target concentrations in the micro-molar range. Therefore, a novel cancer biosensor on the basis of = microRNA-181a biomarker was proposed which the peak currents are related to the concentration of the target oligonucleotide sequence. |
| کلمات کلیدی | MicroRNA-181a, Cancer, Electrochemical Biosensor, Electropolymerization, and polypyrrole. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Scan Nov 25, 2020 (1).pdf | 1399/09/05 | 731115 | دانلود |
| Nikipar.docx | 1399/09/05 | 1305521 | دانلود |
