چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| میلاد حسن پور | اول |
| رضا رهبرقاضی | دوم |
| علیرضا نورآذریان | سوم |
| محمد ولیلو | چهارم |
| شمس الدین ساعدموچشی | پنجم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | بیماری آلزایمر، آمیلوئید بتا، سلول بنیادی مزانشیمی |
| خلاصه مقاله | Background: Alzheimer's disease (AD) is one of the most common neurological disorders, with clinical signs of selective cognition, especially those associated with decline in memory. One of cause of AD is the deposition of beta amyloid (Aβ) plaques and neurofibrillary tangles (NFTs) in the brain, that's the result by synaptic dysfunction and neurodegeneration. Methods: The amniotic fluid mesenchymal stem cell conditioned medium (AF-MSCs-CM) sample was collected from the placenta with the conscious consent of the Pregnant mothers. Condition media were then used to treat 5 to 12 passages of cells. we then treated the sh-sy5y cells. And the levels of ACE and MMP9 enzymes were evaluated. Results: When cells SH-SY5Y are treated with AF-MSC, enzymes ACE and MMP9 increase the act PCR experiments showed that some genes involved in neuronal migration had a significant and significant increase at a concentration of 10. And there was an acceptable increase in the cell differentiation category, but not as much as in the previous category. In synaptic functions, there were results such as cell differentiation. There was a significant increase in growth factors and cytokines and showed that at this concentration 10LPS caused the expression of genes. There was little expression in apoptosis, cell adhesion and cell cycle. We had relatively good expression in signal converters, transcription factors and cofactors compared to the control group. These data show that at a concentration of 10LPS in this cell line used, we saw an increase in the expression of different genes.ivity compared to the control group. Conclusion: According to various studies on the therapeutic potential of AF-MSCs-CM secretions on various diseases and due to the fact that to date no study has been conducted on the potential therapeutic effects of AF-MSCs-CM secretory factors on AD, so in this project aims to determine the effects of these factors on Aβ degrading enzymes and calpain1 protein in the cellular model of AD, which can be used in the future treatment of AD if the desired result is achieved. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| govahi poster.jpg | 1399/08/28 | 486775 | دانلود |
| 20.docx | 1399/08/28 | 36778 | دانلود |
| 1(1).docx | 1399/08/30 | 15234 | دانلود |
| 2.docx | 1399/08/30 | 16992 | دانلود |
| 550.pdf | 1399/08/30 | 465752 | دانلود |
| 1-20.pdf | 1399/08/30 | 2813270 | دانلود |
