چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Developinga simpleand fastmethodfor quantificationofvitaminsincomplex matrixesisone ofthe important issuesinquality control of foods such as milk. In this study, after introducing a new method for quantification of vitamin E in infant formula, its content was compared to the label claims. In this study, dispersive liquid-liquid microextraction (DLLME) procedure with acetonitrile and chloroform as dispersive and extraction solvents, respectively, was used for isolation and clean-up of vitamin E from infant formula samples without the need for saponification. Then, reversed-phase high-performance liquid chromatography(RP-HPLC)composedofaC18columnasstationaryphaseandthemixtureofacetonitrile,methanol,andwater(91:8:1%) asmobilephase usinga standardadditionmethodwas employedfor quantificationofvitamin E (α-tocopheryl acetate)withUV detection at 296 nm. After method validation under the optimum conditions, the method provided a linear range with a determination coefficient (R2) of 0.99 and acceptable accuracy and precision. Results showed that the developed method is an appropriate method for quality control of infant formulas. The advantage of DLLME compared with saponification process and liquid-liquidextractionisthedecreaseoforganicsolventconsumptionandproposingofasimpleandfastmethodforanalysisof vitamin E in infant formula. Application of the developed method for analysis of vitamin E in infant formulas on the market showed there was a considerable difference between labeled and obtained content in most of the samples. |
| نویسنده | نفر چندم مقاله |
|---|---|
| فاطمه صدری کیا | اول |
| علی شایان فر | دوم |
| هادی ولیزاده | سوم |
| محبوب نعمتی | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Food Analytical Methods (2019).pdf | 1399/08/08 | 1116063 | دانلود |
