چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | امیر فتاحی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | سلامت باروری زنان |
| کد مقاله | 74277 |
| عنوان فارسی مقاله | Fertility Preservation: Optimization of Follicle Extraction before Cultivation in the Artificial Ovary |
| عنوان لاتین مقاله | Fertility Preservation: Optimization of Follicle Extraction before Cultivation in the Artificial Ovary |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | (conference of the German Society for Gynecology and Obstetrics (DGGG |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Germany |
| شهر محل برگزاری کنگره/ همایش | Munich |
| سال انتشار/ ارائه شمسی | 1399 |
| سال انتشار/ارائه میلادی | 2020 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1399/07/16 الی 1399/07/19 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | https://www.thieme-connect.com/products/ejournals/abstract/10.1055/s-0040-1717705 |
| آدرس علمی (Affiliation) نویسنده متقاضی | Tabriz University of Medical Sciences, Women's Reproductive Health Research Center, Tabriz, Iran, Islamic Republic |
| نویسنده | نفر چندم مقاله |
|---|---|
| امیر فتاحی | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Objective To maintain fertility before gonadotoxic therapy, ovarian tissue can be frozen and transplanted again after therapy, but not in patients with a medium / high risk of ovarian metastasis. The artificial ovary is a promising alternative, because the ovarian follicles are extracted from the tissue and thus separated from malignant cells. An essential factor in the development of the artificial ovary is a protocol that extracts as many ovarian follicles as possible intact and capable of survival. Material and methods A comparative analysis of purely mechanical and combined mechanical-enzymatic extraction methods of porkine ovarian follicles was carried out, whereby the concentrations of the two enzymes used (collagenase, liberase) and the enzyme treatment protocol used were varied. To evaluate the viability, intact follicles were cultivated for 10 days with a subsequent live / dead assay for vitality analysis [ Figure 1 ]. [1] Results The number of intact follicles was higher when using collagenase (421) than with Liberase (393). The viability of the follicles was higher for the Liberase extraction: after 10 days of cultivation, 92.45% of 138 follicles with Liberase extraction were vital (> 90% living granulosa cells) and 82.14% of 127 follicles with collagenase extraction. Intact follicles could not be extracted with purely mechanical extractions. Summary A combination of mechanical and enzymatic (0.5 mg / ml Liberase concentration) extraction proved to be the most productive extraction method. The protocol determined can serve as a basis for in-vitro and in-vivo testing of the artificial ovary |
| کلمات کلیدی | fertility; Ovary; Follicle |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract in congress.jpg | 1399/08/06 | 294179 | دانلود |
| Poster certificate.jpg | 1399/08/06 | 53272 | دانلود |
| meeting abstract.JPG | 1399/08/08 | 121343 | دانلود |
