چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Purpose: The expression of miR-146a-5p and miR-193a-5p in colorectal cancer (CRC) is associated with cancer development, metastasis, and reduced survival rate of the tumor-suffered subjects. This examination aimed to assess the impact of these microRNAs (miRNAs) in CRC and their mechanisms in the proliferation and migration of cancer cells. Methods: miR‐146a‐5p and -193a-5p were transfected into the HT-29 cell line and assessed their impact on metastasis-related genes. The synergistic effects of these miRNAs on migration were evaluated by wound healing approach. To assess the influence of these miRNAs on the proliferation of and apoptosis of cells, the MTT test, annexin V staining test, and DAPI staining test were done. Then, the protein expression of extracellular-signal-regulated kinase (ERK) and phosphorylated ERK (p‐ERK) were investigated. Results: miR‐146a‐5p and-193a-5p could inhibit the colorectal cancer cells proliferation, and could synergistically induce apoptosis in CRC cells, and also repressed cell migration, and could reduce p-ERK expression. Conclusion: miR-146a-5p and-193a-5p have an important role in cell viability and proliferation via ERK signaling pathway. Thus, the simultaneous use of these miRNAs may be suggested as a probable therapeutic strategy in this cancer therapy. |
| نویسنده | نفر چندم مقاله |
|---|---|
| سعید نورعلیایی | اول |
| الهام باغبانی | دوم |
| داریوش شانه بندی | سوم |
| وحید خاضع شاهگلی | چهارم |
| امیر باغبانزاده | پنجم |
| بهزاد منصوری | ششم |
| خلیل حاجی اصغرزاده | هفتم |
| امیر علی مختارزاده | هشتم |
| بهزاد برادران | نهم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Synergistically Inhibited the Proliferation.pdf | 1399/07/14 | 1610381 | دانلود |
| Rezayat nameh2.jpeg | 1399/07/15 | 1335773 | دانلود |
