چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| In present work, an efficient, reliable and accurate analytical method has been used for the extraction of five organophosphorus pesticides (diazinon, chloropyrifos, phosalone, parathionmethyl and azinphos-methyl) from milk samples. The extracted analytes were determined with high performance liquid chromatography-diode array detector. For this purpose, the milk proteins are precipitated by trichloroacetic acid and the analytes dissolved in the supernatant phase are adsorbed onto an octadecyl silane sorbent. Then, acetone is contacted with the sorbent to elute the analytes and then it is mixed with toluene. The mixture is rapidly dispersed into deionised water filled into a syringe. To break down the obtained cloudy solution, 250 µL acetonitrile is added to the solution as a demulsifier. Finally, the collected organic phase in the tip of the syringe is collected, evaporated and reconstituted in mobile phase for using in determination of the analytes. Figure of merits were calculated for method validation and acceptable limits of detection (0.17–0.36 ng mL–1) and quantification (0.57–1.34 ng mL–1), high enrichment factors (395–460) and extraction recoveries (79–92%) were obtained. Inter- (n = 6) and intra-day precisions at a concentration of 3 ng mL–1 (each analyte) with relative standard deviations ≤7.2%, was obtained. Lastly the method was successfully used for determination of the analytes in some milk samples and chloropyrifos was found in one sample. |
| نویسنده | نفر چندم مقاله |
|---|---|
| محمدرضا افشار مقدم | دوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| zeiadi khandaghi.pdf | 1399/07/14 | 4774347 | دانلود |
