چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Purpose:Non-coding RNAs play a critical role in gene regulation in cancer cells. Reduced expression of microRNA-192 has been detected in many cancers. Here, we investigated the role ofmiR-192in Dihydrofolate reductase(DHFR) andThymidylate Synthase(TYMS) expression level and in an acute lymphoblastic leukemia cell line. Basic procedures:20 patients diagnosed with acute lymphoblastic leukemia (ALL) were studied for the level of (micro RNA-192)miR-192, DHFRandTYMSexpression level by qRT-PCR. NALM-6 cells were transduced using recombinant lentiviruses for overexpression ofmiR-192and its backbone, then the relative changes inDHFRand TYMSgenes expression were studied by qRT-PCR.DHFRProtein level changes were analyzed by Western blotting. Main findings:ALL patients with relapse, experience lower levels ofmiR-192and higher levels ofDHFRand TYMSin comparison with treated patients. Overexpression of miR-192in NALM-6 cells resulted in decreased expression ofDHFRandTYMS. Moreover, the protein level of DHFRwas decreased after overexpression ofmiR-192. Principal conclusions:These results suggest the tumor suppression effects ofmiR-192and its correlation with decreasedDHFRandTYMSlevel in acute lymphoblastic leukemia cells for the first time |
| نویسنده | نفر چندم مقاله |
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| مهدی عدالتی | چهارم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
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| Edalati et al 2020.pdf | 1399/07/12 | 2981971 | دانلود |
