چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Leishmania parasites recognition is very prominent in clinical studies of leishmaniasis and its diagnosis. Though there are various clinical and epidemiological approaches to identify Leishmania infantum, due to some limitations of traditional methods, sensitive and specific techniques are needed and highly demanded. For achieving selective and rapid detection method, a sensitive signal transducer with high surface area is necessary. In this work, a new paper sensor was fabricated using silver nanoprism electrodeposited on the GQDs conductive nano ink (Ag NPr/GQDs nano-ink). The high surface area and the creation of a suitable interface for the anchoring of biomolecules was achieved by electrodepositing of gold nanoparticles (Au NPs) operationalized by cysteamine (AuNPs-CysA) on the superficies of the paper sensor altered by Ag NPr/GQDs nano-ink. To prepare a sensitive and selective bio-device for the recognition of Leishmania in human plasma specimens, DNA-thiol probe was stabilized on the superficies of platform. Hybridization of DNA was evaluated by chronoamperometry (ChA) technique. Engineered DNA-based paper biosensor showed high sensitivity and selectivity for identification of Leishmania genomic DNA. Under optimum circumstances, the linearity range was obtained using photographic paper and ivory sheet 1 µM to 1 zM and 1 nM to 1 zM, respectively. Also, the lower limit of quantitation (LLOQ) in photographic paper and ivory sheet were 1 zM. In addition, the designed DNA-based biosensor revealed well-defined performance to recognition of mismatched sequences (single base, two base and three base mismatches) and selectivity evaluation of the engineered biosensor. |
| نویسنده | نفر چندم مقاله |
|---|---|
| فاطمه فرشچی | اول |
| آرزو سعادتی | اول |
| محمد حسن زاده محله | سوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 1.pdf | 1399/07/10 | 6885078 | دانلود |
