چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مریم محمودزاده مغانجوقی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | دانشکده تغذیه |
| کد مقاله | 73298 |
| عنوان فارسی مقاله | اثر نگهداری درشرایط یخچالی روی بیان ژن های شیگاتوکسین اشرشیاکلی O157:H7 در گوشت چرخ شده |
| عنوان لاتین مقاله | Refrigerated storage effect on expression of shiga toxin genes in Escherichia coli O157:H7 in ground meat |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | بیست و یکمین کنگره میکروب شناسی ایران |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1399 |
| سال انتشار/ارائه میلادی | 2020 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1399/05/27 الی 1399/05/30 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://ismcongress.ir/ |
| آدرس علمی (Affiliation) نویسنده متقاضی | Nutrition Research Center and Department of Food Science and Technology, Faculty of Nutrition and Food Science, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| مریم محمودزاده مغانجوقی | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Purpose: Escherichia coli O157:H7, the main agent of hemorrhagic colitis, produces shiga toxins as key virulence factors. Investigation of pathogenicity of bacteria in broth or broth like modes doesn’t match properly with results obtained from food matrix. So in this work we investigated stx genes expression of E. coli O157:H7 expression in ground beef matrix during 7 days. Methods: Comparative real-time RT-PCR was used to evaluate stx1A and stx2A genes expression of E. coli O157:H7 against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference gene. Results: Expression of both of the stx genes decreased during storage of ground meat. Expression reduction was progressive during storage but there were no significant differences between different days. So the highest reduction was observed on the last evaluation day (day7) that was -3.35 and -2.9 for stx1A and stx2A, respectively. Conclusion: It seems E. coli O157:H7 possess low risk in prolonged storage in ground beef in terms of stx genes expression. However there are different reports of stx genes expression of E. coli O157:H7 in various food matrixes such as lettuce, milk, fish and fruits. So it could be concluded that several factors are influencing on growth and pathogenicity of bacteria in different mediums and generalizing of results obtained in broth media to food matrix should not be always correct. However in this study it is not obvious that is gene expression reduction is equal to reduction in toxin release? |
| کلمات کلیدی |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Ism_certificate-1.pdf | 1399/06/01 | 198673 | دانلود |
| Microbiology congress.docx | 1399/06/01 | 13170 | دانلود |
| ISMCD_Book.pdf | 1399/06/10 | 4029651 | دانلود |
