چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| هانیه صالحی پورمهر | اول |
| نسرین ابوالحسن پور | دوم |
| سکینه حاج ابراهیمی | سوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Introduction and Objective Neurogenic bladder dysfunction (NGB) has an impact on the quality of life. Stem cell (SC) transplantation in the management of neuro-urological diseases is accompanied with promising outcomes. The present review investigates the effect of SC therapy on bladder functional recovery after the onset of SCI, MS, PD, and stroke in rodent models. Materials and Methods A systematic search was conducted in May 2018. All relevant preclinical studies on rodents (e.g. mouse and rat) involving SCI, PD, MS, and stroke-induced-NGB that performed SC transplantation in the management of NGB, and reported detailed urodynamic findings were included. We excluded all studies with human subjects or in vitro. Two authors independently recorded the information by using a data extraction form and also, assessed the methodological quality of the selected trials. The internal validity of the enrolled studies (e.g. selection, performance, detection and attrition bias) and other study quality measures (e.g. reporting quality, power) were assessed using a modified version of the CAMARADES' (Collaborative Approach to Meta-Analysis and Review of Animal Data from Experimental Studies) study quality checklist. We registered the title of the present study in http://joannabriggs.org/research/registered_titles.aspx. Results From 121 relevant publications, 21 studies met our inclusion criteria of which 15 were conducted on SCI, 3 on PD, 2 on stroke and one on MS in the rodent models. We conducted a meta-analysis on SCI experiments and for other neurological diseases, detailed urodynamic findings were considered. A flow chart for data selection is represented in Figure 1. Most of the publications used rats and only three studies related to SCI and MS experiments in the model of mouse. In 4 publications, male rats were examined while the others were conducted on female rats, and gender was not mentioned in two publications. The common delivery routes for cell transplantation were intra-thecal, intra-bladder wall or intratail vein, substantia nigra and medial forebrain bundle. In SCI, 9 studies met all inclusion criteria to analyse and eventually were included in our meta-analysis. The common SC sources used for therapeutical purposes were neural progenitor cells, bone marrow mesenchymal SCs, human amniotic fluid SCs, and human umbilical cord blood SCs. There was a significant improvement of micturition pressure in both contusion and transaction SCI models 4 and 8 weeks post-SC transplantation. Residual urine volume, micturition volume, and bladder capacity were improved 28 days after SC transplantation only in the transaction model of SCI. Non-voiding contraction recovered only in 56 days post-cell transplantation in the contusion model. According to the Begg and Mazumdar’s correlation, we found no publication bias in the meta-analysis. CAMARADES' study quality checklist contains details notably randomized allocation (model/sham groups), blinded induction of the model and assessment of outcomes, calculation of the sample size, compliance with existing animal welfare act, the disclosure of all relevant conflicts of interest, reporting of animal exclusions, and publication in peer-reviewed journals. All articles had been issued in peer-reviewed journals. Only 33.3% studies had disclosure statements for conflicts of interest. The all papers except one stated compliance with the animal protection act. The procedure of random assignment to the groups was seen in 25% of studies. All selected articles declared blinded induction of the animal model. No study existed to state the methodology of sample size calculation and only six studies illustrated both animal exclusion criteria and blind outcome assessment Conclusion The present systematic review and meta-analysis regarding bladder recovery after SC therapy in SCI demonstrate significant improvements of micturition pressure after SC transplantation in both contusion and transaction models in two-time points i.e. 4 and 8 weeks. Residual volume and bladder capacity improved after treatment only in the 28 days after transplantation in the transaction model of SCI. Non-voiding contraction recovered only in 56 days’ post-transplantation in the contusion model. Considering the application of SC in SCI preclinical studies, high quality experiments to reduce the potential risk of bias are necessary for improved understanding of bladder recovery. Also due to limited studies on the relation of other neurological disorders such as PD, stroke, and MS with NGB, additional studies with the modified methodology to reduce the risk of bias are needed to prove the underlying mechanism and to achieve an appropriate approach for bladder recovery. Concluding message Partial bladder recovery has been evident after SC therapy in SCI models. Additional studies in the other neurological diseases with a modified methodology to reduce the risk of bias are needed to confirm the detailed mechanism for bladder recovery. |
| کلمات کلیدی |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| certificate1.pdf | 1399/05/31 | 259529 | دانلود |
| certificate1.pdf | 1399/05/31 | 259529 | دانلود |
