چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Allergic asthma is a T helper (Th) 2 immunological disorder with consequential uncontrolled inflammatory responses. There is an increasing demand to use new methods for the treatment of asthma based on modulation of the Th2-to- Th1 ratio in favour of the Th1 population. Accordingly, we decided to evaluate the effects of intratracheal administration of Kit+ bone marrow cells on tracheal responsiveness and the expression of Gata3 and Tbx21 genes. Forty maleWistar rats were allocated randomly into four experimental groups: healthy rats (control group), sensitized rats (OVA group), sensitized rats receiving Kit− cells (OVA+Kit− group) and sensitized rats receivingKit+ cells (OVA+Kit+ group). Total and differentialwhite blood cell counts, tracheal responsiveness to cumulative methacholine concentrations and histopathological analysis were evaluated. The results showed a statistically significant increase in total white blood cell, eosinophil and neutrophil counts, tracheal contractility, Gata3 expression and prototypical histopathology of asthma. Along with these conditions, we found that the number of lymphocytes was decreased and expression of Tbx21 diminished in sensitized rats compared with control animals. Monitoring of labelled tagged cells confirmed successful engraftment of transplanted cells in pulmonary tissue. Juxtaposition of Kit+ cells changed the blood leucogram closer to the control values. Kit+ cells increased the expression of Tbx21 and suppressed Gata3 (P < 0.05). In the OVA+Kit+ group, tracheal responsiveness was improved coincident with increased pulmonary regeneration. In conclusion, this study showed that intratracheal administration of bone marrow-derived Kit+ cells, but not Kit− cells, could be effective in the alleviation of asthma, presumably by the modulation of Gata3 and Tbx21. |
| نویسنده | نفر چندم مقاله |
|---|---|
| حسین حیران | اول |
| مهدی احمدی | دوم |
| رضا رهبرقاضی | سوم |
| فاطمه میرارشادی | چهارم |
| عارف دلخوش | پنجم |
| مجید خاکسار | ششم |
| مرتضی حیدرزاده | هفتم |
| رعنا کیهان منش | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Ckit-t-bet-GATA.pdf | 1399/06/14 | 6942039 | دانلود |
| Dr. Ahmadi\'s letter.docx | 1399/06/16 | 65148 | دانلود |
