| Abstract The aim of the study was to investigate the effect
of the extracted egg yolk antibody along with lycopene on
the chemical quality of the rainbow trout fillet during
16 days of refrigeration storage. Chickens were immunized
against Pseudomonas fluorescens (P. fluorescens), Shewanella
putrefaciens (S. putrefaciens) and total spoilage
bacteria and their eggs were collected for the isolation of
egg yolk antibodies. Then fish fillets were immersed in
chitosan-based coating solutions, containing lycopene and
extracted antibodies, and analyzed for lipid oxidation
changes (peroxide, thiobarbituric acid, free fatty acid and
fatty acid profile), physico-chemical properties (pH and
water holding capacity), and sensory evaluation, during
16 days of refrigeration storage. Results showed that chitosan
solutions with lycopene or IgY could significantly
(p\0.05) increase the oxidative stability of lipids in fish
fillets; although, combinational use of lycopene and IgY
showed a higher effect on delaying the rate of lipid oxidation.
Significant differences were also observed between
treatments contained combination of chitosan, antibody
and lycopene with the control group, regarding pH and
WHC. Saturated fatty acids increased in all treatments,
although the changes in the treatments containing lycopene
and antibody were significantly (p\0.05) lower than the
control group. Hence the addition of egg yolk antibody and
lycopene in coating solution are good bio-preservatives for
seafood products as it improves sensory attributes and
prevents lipid oxidation. |