چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| هانیه صالحی پورمهر | اول |
| سکینه حاج ابراهیمی | سوم |
| امیررضا ناصری | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | stem cells, urinary bladder, spinal cord injury |
| خلاصه مقاله | Background and aim Neurogenic bladder (NB) is a major global medical problem that affect the patient`s quality of life all around the world. Central or peripheral nervous system injury following many neurological diseases leads to the bladder dysfunction. Stem cell transplantation is a new approach to treat many diseases such as bladder dysfunction may overcome to the limitation of current therapies. We aimed to investigate the effects of autologous bone marrow mesenchymal stem cells transplant following experimental spinal cord injury on urinary bladder morphology. Methods A total of forty-two female Wistar rats were randomly divided into 6 groups (7 in each). A laminectomy was performed at the T9–10 level to expose one spinal cord segment. In the hemisection or complete transection model, either a T9 spinal cord hemisection on the left of the spinal cord or a T9 transection was performed with a sharp blade visually. Four weeks after spinal cord injury (SCI) induction, 1*106/10µl of rat BMSCs were injected into the six areas of bladder wall in two groups. Animals were sacrificed on day 28 after transplantation. Bladder tissues were taken and fixed with 4% formalin solution. Then, the tissues were paraffin embedded. 4-µm thick slides were prepared from the bladder sections. To measure the content of collagen fibers in histological slides from the urinary bladder, samples were subjected to Masson’s Trichrome staining. The intensity of bladder wall fibrosis was evaluated as follows: Grade 0 (none) – without fibrosis and muscular hypertrophy; grade 1 (mild) – without muscular hypertrophy, only thin fibrotic tissue was observed between the epithelium and the lamina propria; grade 2 (severe): muscular hypertrophy and continuous fibrotic tissue was observed among the epithelium, the lamina propria, and the muscularis propria. Result Histological examination revealed that the urothelium and lamina propria thickness was higher in the injured rats following SCI in comparison with the control or sham operation. The distributions of bladder muscle (red stain) and fibrous tissue (blue stain) were analyzed by the MT stain that showed that in SCI group, collagen deposition in the bladder increased markedly in the SCI group compared with the control or sham groups. After treatment, these changes were decreased significantly in hemisection SCI group only (p=0.042). Table 1 represent the values of bladder wall fibrosis in all groups. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| photo_2020-06-02_23-26-20.jpg | 1399/03/13 | 141063 | دانلود |
| abstract.doc | 1399/03/13 | 37376 | دانلود |
| ANSWER.docx | 1399/03/26 | 171317 | دانلود |
