چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Owing to the role of fractalkine in regulating cellular apoptosis/proliferation, we investigated fractalkine efects on apoptosis/ proliferation signaling of granulosa cells in polycystic ovarian syndrome (PCOS) patients through in vitro and in vivo experiments. In vivo, granulosa cells were collected from 40 women undergoing oocyte retrieval (20 controls and 20 PCOS). The expression levels of fractalkine, BAX, Bcl2, Bcl2-XL, Bad, and TNF-α were assessed using RT-PCR. In vitro, we determined the efect of diferent doses of fractalkine on the expression of the above mentioned genes in GCs of both groups. We found that the expression levels of fractalkine and Bcl-2 were signifcantly lower in the GCs of PCOS patients compared to the control group (p<0.05). In contrast, the expression levels of TNF-α and BAX were higher in the patient’s group than in the control group. The results suggested that expression levels of fractalkine were negatively and positively correlated with the number of oocytes and fertilized oocytes respectively. Moreover, fractalkine could dose-dependently increase fractalkine and decrease BAD, BAX, Bcl-xl, and TNF-α expressions in the control GCs. In contrast, GCs collected from PCOS patients revealed an increase in expression of BAD, BAX, and Bcl-xl following fractalkine treatment. Our fndings indicated that insufcient expression of fractalkine in PCOS patients is related with elevated apoptotic and infammatory markers and reduced anti-apoptotic genes in the GCs. |
| نویسنده | نفر چندم مقاله |
|---|---|
| آیدین راعی صدیق | اول |
| مسعود دارابی امین | دوم |
| علی سلماسی | سوم |
| کبری حمدی | چهارم |
| لعیا فرزدی | پنجم |
| عالیه قاسم زاده | ششم |
| امیر فتاحی | هفتم |
| محمد نوری | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Fractalkine.pdf | 1399/02/18 | 2274143 | دانلود |
