چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Antibodiesarestillwidelyusedinseveralprogramsincludingearlyresearch,imaging,Targetingdrugdeliverysystem, Affinity chromatography, flowcytometry technic, diagnosis and treatment. Purification of antibody is a standard approach for detectionofinfectionagentindifferentspecies.ThereservoirhostsforleishmaniainfantumareDogsandtheyhaveactiverolein thetransmissionofleishmaniatohumansbythebiteofasandflybelongingtogenusPhlebotomusandLutzomiya.Consequently, elimination of dogs in endemic areas and vaccination of dogs contributes to reduction of the human and canine VL cases. Serological antibody tests such as IFAT (Indirect Fluorescent Antbody Test), DFAT (Direct Fluorescent Antbody Test), ELISA (Enzyme–Linked Immunosorbent Assay), PCR (Polymerase chain Reaction Assay) have been extensively used to investigate canine infection with L. infantum. In this study we produced and purified polyclonal antibody against attenuated and wild type leishmania infantum in dogs. Anti-leishmania in dog serums precipitated with ammonium sulphate. The IgG recovered from ammonium sulphate precipitation was subject to ion exchange chromatography (IEC) and the purity of IgG was confirmed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) under reduced condition. The purity of proteins were above 95% and then purified IgG was conjugated with FITC. We determined optimum titer of dog IgG by observation parasites underfluorescentmicroscope.TheoptimumdilutionofpreparedFITCconjugateddogIgGwas1:400.Thispolyclonalantibody can be used for other applications in research, diagnosis and clinic. |
| نویسنده | نفر چندم مقاله |
|---|---|
| جعفر مجیدی ذوالبین | دوم |
| جلال عبدالعلیزاده | چهارم |
| لیلی عاقبتی | پنجم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Untitled.png | 1399/07/08 | 65976 | دانلود |
| Production.pdf | 1399/07/08 | 1306506 | دانلود |
