چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Magnetic Fe3O4 nanoparticleswere synthesized successfully by co-precipitation method and characterized using XRD, EMand EDS analyses. Then doxorubicin (DOX, a known anticancer drug)was loaded onto nanoparticles. In vitro DNA interaction of free DOX and loaded DOX onto Fe3O4 nanoparticles (DOX-Fe3O4) was investigated by DNA- iscositymeasurements, UV–visible and fluorescence spectroscopies. The obtained values for binding constant ofDOX andDOX-Fe3O4 compounds fromUV–visible spectroscopieswere 0.04× 105 and 0.68× 105Lmol−1, respectively, which confirms DOX-Fe3O4 compound have a stronger interaction with CT-DNA compared to DOX. Considerable changes on viscosity of the compounds recommended that their binding mode with CT-DNA is intercalative binding. Fluorescence intensity of DOX and DOX-Fe3O4 was quenched via static process by regular addition of CT-DNA. Thermodynamic parameters suggest that Van der Waals forces and hydrogen bonding for DOX and electrostatic forces for DOX-Fe3O4 are predominantly responsible for interaction with CT-DNA. Competition fluorescence studies were done by Hoechst 33258 as a well-known groove binder and ethidium bromide (EtBr) as a known intercalator probe. Percentage of displacement for EtBr-DNA complex with DOX and DOXFe3O4 was 39% and 61%, and for Hoechst-DNA complex was 9% and 5%, respectively. These results confirmed that both compounds are intercalator binders, although DOX-Fe3O4with a further 22% displacement is a stronger intercalator binder than DOX. The stronger interaction of DOX-Fe3O4 compared to DOX suggests that the current system can be used as a new and effective way to targeted therapy of anticancer drug |
| نویسنده | نفر چندم مقاله |
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| زینب میرزایی کالار | اول |
| ابوالقاسم جویبان | سوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Spectrochim Acta A 229 (2020) 117985.pdf | 1399/02/02 | 1842599 | دانلود |
