چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Antibodies are used in many diagnostic and experimental procedures. The primary goal in experimental polyclonal antibody production is to obtain sufficient volumes of high titer and high-affinity antibodies economically. Anti-rabbit Immunoglobulins and its conjugate with Horse Radish Peroxides (HRP) is used to diagnosis rabbit diseases by ELISA or western blotting tests. In this study, the production, purification and HRP conjugation of polyclonal IgG against rabbit Immunoglobulins in rats were carried out. Five 6-month-old Male Wister rats were immunized by rabbit Immunoglobulins in combination with Freund’s adjuvant. Purified antibody (using ion-exchange chromatography) was labeled to HRP. Direct enzyme linked Immune-sorbent assay (ELISA) was used to determine the optimum titer and cross reactivity of HRP conjugated IgG. The purity of various IgG preparations was about 98%. The optimum dilution of prepared HRP conjugated IgG was 1:25600. This conjugated IgG has no cross reactivity with guinea pig and hamster Immunoglobulins at optimized dilution. The result of the present study showed that ionexchange chromatography could be considered as an appropriate method for purification of IgG antibodies. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مسعود مقدس زاده | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Moghadaszadeh2014.pdf | 1399/01/25 | 182950 | دانلود |
