چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Influenza can be controlled by vaccination but the virus due to its high mutation rate, has limited efficacy. Influenza viruses possess conserved epitopes matrix-2 protein ecto-domain (M2e) and HA2 that could be used in a vaccine. In this work, M2e-HA2 peptide was expressed in E. coli and then purified. The M2e-HA2 gene from influenza A virus was then synthesized and cloned into pGS21vector. The construct was transferred into E. coli BL21 (DE3) and induced using IPTG. Expression of recombinant peptide was confirmed by western blot assay using anti - GST monoclonal antibody. The expressed peptide-GST was purified from bacterial lysate by IMAC chromatography. The resulting sequence revealed that the M2e-HA2 gene was cloned in the vector. SDS-PAGE electrophoresis of purified peptide demonstrated a strong single band. Western blot analysis showed a single band in correct position. The purified peptide could be tested In vivo against influenza virus infection. |
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| مسعود مقدس زاده | اول |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
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| Moghadaszadeh 2015(1).pdf | 1399/01/25 | 310242 | دانلود |
