چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Abstract Objectives: MicroRNAs are small (19–23nt) noncoding RNAs that adjust gene expression post-transcriptionally. In addition, microRNA (miRNA) profile modification is revealed in various abnormalities including cancer. Formalin-fixed paraffinembedded (FFPE) material archives are valued for searching human diseases. Therefore, the present study aimed to investigate FFPE samples for miRNA expression, as an alternative analyte for gene expression with the growing therapeutic and diagnostic potential. Further, the validity of RNA isolation kits was evaluated in order to identify a preferred method. Materials and Methods: The two extracted methods were qualitatively and quantitatively assessed in order to compare the archived specimens between these methods. The total RNA and gene expression were quantified in each method as well. Results: Based on the results, the level of RNA extracted from FFPE tissues and real-time polymerase chain reaction (RT-PCR) of hsa-miR-623, hsa-miR-515-5pin miRNeasy FFPE (P value<0.047) was significantly higher than that in RNAX Plus (P<0.084). Conclusions: In general, the spin column method for miRNA expression profile had better expression compared to the noncolumn method. The results further confirmed the validity of FFPE tissues as an appropriate resource for miRNA analyses. |
| نویسنده | نفر چندم مقاله |
|---|---|
| زهرا آصفی | اول |
| علیرضا ابهری | دوم |
| بهروز شکوهی گوگانی | سوم |
| زینب لطیفی | چهارم |
| سعید نظری سلطان احمد | پنجم |
| ناصر صمدی | هفتم |
| محمد نوری | هشتم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| pdf_CJMB_317.pdf | 1399/01/25 | 195259 | دانلود |
