چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Behçet's disease (BD) is an autoinflammatory disease caused by several genetic, epigenetic, immunologic, and environmental factors. This study aimed to evaluate the association between CTLA4 gene +49A/G polymorphism and risk as well as clinical manifestations of BD in the Iranian Northwest Azeri population. In this study, 47 BD patients and 61 healthy controls were enrolled. CTLA4 gene +49A/G genotyping was conducted via the tetra-primer amplification refractory mutation system–polymerase chain reaction (T-ARMS–PCR). CTLA- 4 gene expression was determined by Real-time PCR and the serum level of CTLA-4 protein (sCTLA-4) was evaluated by ELISA. The A allele (OR = 3.133, 95%CI = 1.53–5.59, P = 0.005) and AA genotype (OR = 5.54, 95% CI = 2.48–13.10, P < 0.001) indicated significantly different frequency between patient and control groups. No statistically significant differences were observed between males and females regarding the genotype or allele frequency. The mRNA expression (P = 0.0002) and serum concentration (P = 0.035) of CTLA-4 were decreased in BD patients in comparison to controls. Moreover, BD patients with AA genotype showed reduced CTLA-4 gene expression (P = 0.0004) and sCTLA-4 concentration (P = 0.02) in patients compared to controls. In addition, AA genotype was associated with the severe clinical manifestations of BD. In conclusion, our study proposes that the AA genotype of the CTLA4 gene +49A/G polymorphism is an important genetic risk factors that can affect BD symptom severity in the Iranian Azari population. But these findings require further studies with larger sample sizes. |
| نویسنده | نفر چندم مقاله |
|---|---|
| ساناز عباسپور اقدم | اول |
| مجید احمدی | دوم |
| امیر مهدی زاده حقیقی | پنجم |
| علیرضا خبازی اسکویی | هشتم |
| لیلا روشنگر | نهم |
| زهره بابالو | دهم |
| امین کامرانی | هفتم |
| یوسف دانشمندپور | چهارم |
| حامد ولی زاده | ششم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abbaspour et al 2020.pdf | 1398/12/21 | 469617 | دانلود |
