چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده ثبت کننده مقاله | مصطفی اکبرزاده خیاوی |
| مرحله جاری مقاله | تایید نهایی |
| دانشکده/مرکز مربوطه | بیماری های گوارش و کبد |
| کد مقاله | 70990 |
| عنوان فارسی مقاله | Oxidative stress-induced antiproliferative and apoptotic effects of nanoformulated RNase A in colorectal cancer cells |
| عنوان لاتین مقاله | Oxidative stress-induced antiproliferative and apoptotic effects of nanoformulated RNase A in colorectal cancer cells |
| نوع ارائه | پوستر |
| عنوان کنگره / همایش | نوزدهمین کنگره بیماری های گوارش و کبد ایران |
| نوع کنگره / همایش | بین المللی |
| کشور محل برگزاری کنگره/ همایش | Iran (Islamic Republic) |
| شهر محل برگزاری کنگره/ همایش | تهران |
| سال انتشار/ ارائه شمسی | 1398 |
| سال انتشار/ارائه میلادی | 2019 |
| تاریخ شمسی شروع و خاتمه کنگره/همایش | 1398/08/15 الی 1398/08/17 |
| آدرس لینک مقاله/ همایش در شبکه اینترنت | http://iaghcongress.org/fa/Service/62aa7539-6079-4af8-acc9-63a58374949a |
| آدرس علمی (Affiliation) نویسنده متقاضی | Liver and Gastrointestinal Diseases Research Center, Tabriz University of Medical Sciences, Tabriz, Iran |
| نویسنده | نفر چندم مقاله |
|---|---|
| مصطفی اکبرزاده خیاوی | اول |
| اعظم صفری | دوم |
| ژاله برار | سوم |
| سید کاظم میری نژاد | چهارم |
| یداله امیدی | پنجم |
| محمد حسین صومی | ششم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Bovine pancreatic ribonuclease; Colorectal cancer; Gold nanoparticles; Nanomedicine; PEGylation; Reactive oxygen species |
| خلاصه مقاله | Introduction: The current investigation introduces an advanced and effective passive targeted tumor therapeutic nanobiomedicine composed of pegylated gold nanoparticles (PEG-GNPs) conjugated with bovine pancreatic ribonuclease (RNase A) that induced reactive oxygen species (ROSs) mediating apoptosis pathway as effective strategies for colorectal cancer treatment. RNase A represents a new class of cytotoxic and non-mutagenic enzymes, has gained more attention as a potential anti-cancer modality, however, the cytosolic ribonuclease inhibitors (RIs) restricted the clinical application of this enzyme. Methods: The GNPs synthesized, stabilized by polyethylene glycol (PEG), functionalized, and covalently conjugated with RNase A. The physicochemical properties of engineered nanobiomedicine (GNPs-PEG-RNase A) were characterized by scanning electron microscope (SEM), dynamic light scattering (DLS), and UV-vis spectrum. Then, its biological impacts including cell viability, apoptosis, and ROS production were evaluated in the colorectal cancer cell line. Results: The engineered nanobiomedicine was found to effectively induced apoptosis in SW-480 cells and resulted in a significant reduction in cancer cells viability. From the flow cytometry results, this nanoscale showed the significant apoptotic effect on cells. In addition, the maximum production of ROS was obtained after the treatment of cells with IC50 dose of GNPs-PEG-RNase A. Conclusion: Based on the efficient ROS-responsiveness and the anti-cancer activity of RNase A of the engineered nanomedicine, this nanoscaled biologics may be considered as a potential candidate for the treatment of colorectal cancer. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| certificate1.pdf | 1398/11/12 | 131839 | دانلود |
| Akbarzadeh -Abstract.docx | 1398/11/12 | 22222 | دانلود |
| Poster.Akbarzadeh.pdf | 1398/11/12 | 903942 | دانلود |
