چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| جلال عبدالعلیزاده | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Probiotics, Apoptosis, Caov-4, AKT, PTEN |
| خلاصه مقاله | Background: Probiotics areliving microorganisms that, with adequate consumption, have good health effectsfor the host. In ovarian cancer, mortality is high, identifying patients indifficult initial stages and resistant to chemotherapy drugs. For thesereasons, the need to develop new options for prevention of ovarian cancer isnecessary. In this study, the effect of lysate, supernatant of Lactobacillus rhamnosus on Caov-4 ovarian cancer cellline was performed. The role of the concentration of canceroous cells treatedwith different concentrations on the results of the MTT assay and theexpression of AKT-PI3K-PTEN genes was investigated. Methods: In this study, bacteria were cultured in specific medium (MRS broth) at anaerobic condition for 24-48 hour. After incubation periodculture medium was centrifuged, separate the supernatant and then the cellswere washed twice with PBS buffer to remove additional medium. Finally, collected bacterial cells disrupted by sonication. The bacteria was cultured in a RPMI 10% without antibiotics, and the was determined optic density at 600 nm.Then was treated with the Caov-4 cancer cell line and prepared media. An MTTtest was performed to determine the toxicity of the treatments on Caov-4. TheExpression of AKT-PI3K and PTEN genes were investigated by RT-PCR. Results: The results indicate theanticancer effects of Lactobacillus rhamnosus, which can be used as apreventive medicine to prevent the development of ovarian cancer. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Effect.pdf | 1398/10/03 | 547741 | دانلود |
| Effect of probiotic bacteria poster.pdf | 1398/10/21 | 368140 | دانلود |
