چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| علی شایان فر | دوم |
| ابوالقاسم جویبان | سوم |
| افشین قره خانی | چهارم |
| فاطمه نوروزی | اول |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Serum concentration of Indoxyl Sulfate, an uremic toxin in Chronic Kidney Disease (CKD) patients Introduction: Indoxyl sulfate, a protein-bound uremic toxin, accelerates the progression of chronic kidney disease (CKD) [1] and is synthesized in the liver from indole, produced from tryptophan by intestinal flora. [2] Despite the wide role of IS in the pathology of renal diseases and cardiovascular events, few methods are available for the quantification of IS in biological fluids and most of them require extensive preparation and long laboratory run time. [3] For highly protein bound drugs, it is also essential to break the bond to allow the accurate measurement of total drug concentration.[4] the goal of this study was to evaluate the serum levels of IS in CKD patients using salting-out assisted liquid-liquid extraction (SALLE) and fluorescence. Methods: In this study 60 serum samples were obtained from CKD patients from sina hospital. they were extracted and deproteinized by the addition of nine part of acetonitrile to one part of serum and centrifuged for 10 min at 8000 rpm. The supernatant was transferred to a microtube and 500 µL of sodium chloride was added. The fluorescence intensity of separated organic phase was determined by a spectroflurimnetry. Results: The affecting parameters on extraction, i.e. volume of solvent, pH, the concentration of salt optimized and the developed method was validated for quantification of IS with a good precision and accuracy (less than 15%). In serum from uremic patients the concentration of indoxyl sulfate was markedly more than that in serum from normal subjects???(P<0.01) Discussion: IS, was thought to play a significant role in the progression of CKD, tubulointerstitial fibrosis, glomerular sclerosis, endothelial proliferation and wound repair were also inhibited by IS.[5] A sensitive and reproducible SALLE extraction method was developed and validated for the determination of IS in plasma. It provides similar simplicity to protein precipitation, but cleaner extracts due to a true phase separation. The precision and accuracy are within the limits required for biological analytical assays. |
| کلمات کلیدی | Chronic kidney Disease patients, Indoxyl sulfate, Salting-out assisted liquid-liquid extraction |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| IMG_5903.PNG | 1398/12/08 | 453777 | دانلود |
| 1.docx | 1398/12/13 | 14675 | دانلود |
| IMG_5946.jpg | 1398/09/24 | 103195 | دانلود |
| IMG_6230.jpg | 1398/09/24 | 79607 | دانلود |
| IMG_6229.jpg | 1398/09/24 | 80449 | دانلود |
