چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| تیمور حضرتیان | سوم |
| اسماعیل فلاح | چهارم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | lran, Leishmania infantum, Phlebotomus, Polymerase chain reaction, Visceral leishmaniasis 201 |
| خلاصه مقاله | Background: Zoonotic visceral leishmaniasis is caused by Leishmania infantum, which is transmitted to humans by bites of phlebotomine sandflies and is one of the most important public health problems in Iran. To detect and identiff the Leishmania parusltes and their corresponding vector(s), an investigation was carried out in Azarshahr County, a new and important focus of the disease in East Azetbaiian province in northwestern Iran during late April to late October 2010. Materials and mthodsi Sandflies were sampled using sticky papers (A4 white paper soaked in castor oil) from inside and outside of the houses and animal shelters, close to the vegetation and crevices. The head and three last abdomen segments of the specimens were removed and mounted in Puri's medium for species identification. The rest of body was subjected to molecular methods for detection of leishmanial parasites. Results and discussion: Among 400 female sandflies tested by polymerase chain reaction (PCR) of kDNA, ITSl-rDNA, and CPB genes of the parasite followed by restriction fragment length polymorphism (RFLP), only 2 out of 8 Phlebotomus tobbi were positiveto L. infantum parasites. Conclusion: The results indicated that, P. tobbi was the only species found infectedby L.iffintum andthe principal vector of the disease agent to human. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract Natural infection.pdf | 1398/09/16 | 661236 | دانلود |
| Certificate Natural infection.pdf | 1398/09/16 | 651378 | دانلود |
