چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| آزیتا دیلمقانی | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | Acute lymphoblastic leukemia, Acrylamide, L-Asparaginase, Optimization |
| خلاصه مقاله | L-Asparaginase (L-asparagine amidohydrolase; EC 3.5.1.1) catalyzes the deamination of l-asparagine in to l-aspartate and ammonia. L-asparaginase exists in various organisms including animals, plants, yeast, fungi, bacteria, and archaea except humans. In recent years due to its important applications, as its use in pharmaceutical industries an alternative for treatment of different cancers such as acute lymphoblastic leukemia, malignant diseases of the lymphoid system and Hodgkin’s lymphomas. Also, this enzyme is used in food industry to prevent the acrylamide formation when foods and maintain the quality of the food. The aim of this study is optimization of L-Asparaginase production of Bacillus subtilis isolated from native honey of Western Iran. For identification, biochemical tests including gram, catalase and oxidase activity, acid and bile salts resistance were done. The 16S rRNA gene sequencing, used to bacterial identification. L-Asparaginase production was evaluated by qualitative (rapid plate assay on phenol red containing media) and quantitative method (Imada method), respectively. Based on the RSM optimization results, the highest amount of enzyme production was 15/50 U/ml. In this condition, the amount of total protein and biomass, respectively 12/3 mg/ml and 80 mg. Based on the results, Bacillus subtilis could be a good candidate for production of asparaginase enzyme. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Tehran.png | 1398/09/14 | 349965 | دانلود |
| 91120987.doc | 1398/09/14 | 43008 | دانلود |
