چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| تیمور حضرتیان | چهارم |
| محمد رحمتی | دوم |
| عنوان | متن |
|---|---|
| کلمات کلیدی | apoptosis, breast cancer, DNA damage, gene expression |
| خلاصه مقاله | Introduction: clofarabine is a purine nucleoside analogue and inhibitor of ribonucleotide reductase enzyme complex (RR) that is used in treatment of blood cancers. Clofarabine-3P binds to RR and inhibits the conversion of NTPs to dNTPs. Low level of dNTP disrupts repairing and replication of DNA that results in induction of p53 and p53R2 subsequently. Purpose: in this study we investigate the effect of clofarabine in induction of apoptosis in P53R2 expression and apoptosis in T47D breast cancer cell line. Materials & Methods: T47D cell line was treated with different clofarabine concentration in three time: 24, 48 and 72h. Then by MTTassay the IC50 of clofarabine was determined. After treating cells, total RNA of them was extracted and their cDNA was built by Reverse Transcriptase enzyme. Then Real Time PCR was done. The Annexine V and PI was used to detection of apoptosis by flow-cytometry method. Result: IC50 for 48 and 72h was determined as 3 and 2.5μM respectively and the 24h treatment hasn’t cytotoxicicity on these cells. Clofarabine induces P53R2 expression in different time treatment of 48 and 72h. In 48h treatment P53R2 gene expression was 1.24 fold compared with control sample (1 fold), for 72h gene expression was 1.77 fold. Also the result of flowcytometry has shown 13.45 and 37.2 percent apoptosis induction for 48 and 72h treatment Conclusion: With regard to the above finding, clofarabine can induces apoptosis through induction of P53R2 in T47D cell line, so it can be considered for more investigation as drug in breast cancer (epithelial cancer cells) treatment. |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| Abstract Clofarabine.pdf | 1398/09/10 | 215408 | دانلود |
| Certificate Clofarabine.jpg | 1398/09/10 | 355589 | دانلود |
