| The methanol extract of the aerial parts of the medicinal plant Pedicularis
sibthorpii Boiss., Scrophulariaceae, growing in the Azerbaijan province of Iran, was
found to be active in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and the antibacterial
agar well diffusion assays, but no general toxicity was observed in the brine shrimp
lethality assay. A combination of solid-phase extraction (SPE) and preparative
reversed-phase high-performance liquid chromatography (prep-RP-HPLC)
analyses of the methanolic extract afforded three phenylethanoids (verbascoside,
martynoside and isomartynoside), an iridoid (aucubin), a flavonoid (luteolin 7-O-
β-D-glucopyranoside) and mannitol, and the structures of these compounds were
elucidated unambiguously by spectroscopic means. The distribution of the isolated
compounds within the genus Pedicularis has also been discussed. |