چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| A novel sandwich-type biosensor based on a signal amplification strategy of various nanocomposites was developed to monitor the prostate-specific antigen (PSA) in real samples. The engineered immunosensor was fabricated using iron oxide magnetic nanoparticles modified with a polymer poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) [Fe3O4/PEDOT:PSS] conductive nanocomposite that utilized the immobilization of primary antibodies (biotinylated antibody (Ab1)). In order to improve the electron transfer rate, a cysteamine-capped gold nanoparticle (AuNPs-CysA) was applied for conjugation with a secondary antibody (HRP-Ab2). The fabrication procedure was investigated by square wave voltammetry. Under optimized experimental conditions, the calibration curve showed a wide linear range (0.001–0.5 μg L−1) for the PSA biomarker with a low limit of quantification of 0.001 μg L−1. Finally, satisfactory results were obtained for the determination of PSA in untreated human plasma samples, indicating the potential of the immunoassay for applications in clinical bioanalysis. |
| نویسنده | نفر چندم مقاله |
|---|---|
| مینا شبان | اول |
| محمد حسن زاده محله | دوم |
| الهام صلحی | سوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| authorreprints.pdf | 1398/08/23 | 2058057 | دانلود |
