چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| فریبا میرزائی باویل | اول |
| رعنا کیهان منش | دوم |
| غلامرضا حمیدیان | سوم |
| زهره زواری اسکویی | چهارم |
| فرزاد بهاری | پنجم |
| میلاد محمدزاده | ششم |
| منیره سیف اله زاده | هفتم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Effect of troxerutin on expression of miRNA192, TGF-β and SIP1 levels of kidney tissue in type 1 diabetic male rats Fariba Mirzaei Bavilو.Rana Keyhanmanesh, Gholamreza hamidian, Zohreh Zavari, Farzad Bahari, Milad Mohammadzadeh, Monireh Seyfollahzadeh Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. Abstract: Background and Objective: Nephropathy is one of the most important complications of diabetes. The increase of TGF-β and miRNA192 levels in diabetic conditions, by suppressing SIP1, increases the level of collagen in the kidney. So, we inspected the effect of troxerutin on cellular processes of nephropathy, from the aspect of the miRNA and its related pathways. Materials and methods: fifty male wistar rats (200-250 g) were randomly divided into 5 groups (n=10): control, control under treatment with troxerutine (150 mg/Kg/day/PO). Diabetic (induced by STZ 55 mg/kg, IP, single dose). Diabetic+ Insulin, under treatment with insulin, (4-6 units/day, NPH). Diabetic+Troxerutin (150 mg/Kg/day/PO) for 4 weeks. At the end, the kidney tissue of all animals were extracted for the measurement of miRNA192, SIP1 and TGF-β. Proteins and miRNA were measured by ELISA and RT-PCR respectively. Results: Diabetes caused a meaningful increase in TGF-β and micRNA192 levels, but troxerutin and insulin decreased them (p<0.05). On the other hand, diabetes considerably decreased SIP1 levels but troxerutin increased it (p<0.05). There was no significant difference between insulin and troxerutin. Conclusion: Diabetes can provoke nephropathy by increasing collagen levels and accumulation of extracellular matrix by the means of increasing the amount of TGF-β and subsequently increasing miRNA192 and inhibiting SIP1. But troxerutin has a reverse effect on this process, and it can probably prevent diabetic nephropathy by increasing SIP1 levels. Keywords: Diabetes, Troxerutin, miRNA192, TGF-β, SIP1 |
| کلمات کلیدی |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| certificat0001.pdf | 1398/08/19 | 891528 | دانلود |
| scan110001.pdf | 1398/08/19 | 1276420 | دانلود |
