چاپ صفحه |  صفحه نخست سامانه |  چکیده مقاله |  نویسندگان |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| Rapid screening of pathogenic microorganisms are widely demand in the last decade. According to fastidious and hard to grow nature, Legionella penumophila is important pathogen for bioassay and biomedical analysis. In this work a novel paper-based genoassay was developed for monitoring of L. penumophila. For the first time, silver-graphene quantum dots (Ag/GQDs) ink was synthesized and used for construction of new substrate for bioassay of L. penumophila. The prepared interface was modified by gold nanoparticles grafted by Cysteamine A (CysA/AuNPs) which is necessary for ssDNA immobilization and hybridization by cDNA. All of the genoassay fabrication steps were characterized by field emission scanning electron microscope (FE-SEM), Energy-dispersive X-ray spectroscopy (EDS), Atomic force microscopic (AFM) and also TEM (transmission electron microscopy). Using chronoamperometry technique, the measurement of target cDNA was performed successfully. Also, cDNA was determined in the linear rang of 1µM to 1ZM which low limit of quantification was 1ZM. The results show that the designed bio-platform despite a simple structure with high sensitivity and specificity for the DNA based bioassay of L. penumophila genome. |
| نویسنده | نفر چندم مقاله |
|---|---|
| احمد موبدکرکج | اول |
| محمد حسن زاده محله | دوم |
| سودابه حسن پور | چهارم |
| آرزو سعادتی | پنجم |
| محمد آقازاده | ششم |
| نسرین شادجو | سوم |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 199.pdf | 1398/08/12 | 1076475 | دانلود |
