چاپ صفحه |  صفحه نخست سامانه |  نویسندگان |  اطلاعات تفضیلی |  دانلود مقاله | دانشگاه علوم پزشکی تبریز |
| نویسنده | نفر چندم مقاله |
|---|---|
| مرجان قربانی | اول |
| لیلا روشنگر | سوم |
| جعفر سلیمانی راد | دوم |
| عنوان | متن |
|---|---|
| خلاصه مقاله | Background and Aim: Cartilage tissue engineering (CTTE) is proposed as an emerging promising therapeutic strategy that offers advantages over the current treatment approaches, which seeks to overcome the cartilage self-repair limitation through the development of cellular scaffolds that closely mimic the complex structure of cartilage tissue. Among a variety of biomaterial scaffolds used in CCTE, in situ injectable hydrogel systems are highly desirable for the clinical applications due to the biocompatibility, highly hydrated 3-D environment similar to the cartilaginous ECM structure, ability to effectively deliver cells and/or bioactive molecules to targeted sites. Methods: About 1.5 g aliquot of pectin was dissolved in 150 mL of purified water in a 500 mL flask. A total of 0.8 g of NaIO4 was dissolved in 10 mL of purified water and added dropwise to the pectin solution. The mixture was allowed to stir for 2 hours at room temperature before 0.4 mL of ethylene glycol was added. The solution was dialyzed for 2 days. The same procedure was used for the oxidation of cellulose CNCs. Finally, a double-barrel syringe was used to make injectable hydrogels of chemically cross-linked pectin and CNCs. Barrel A contained a 4 wt % chitosan solution in purified water and barrel B contained a 4 wt % pectin-CHO and CHO-CNC solution. Then, all materials were sterilized and the polymer solutions at different weight ratios were prepared in the presence of chondrocytes in a specific medium. Subsequently, chondrocytes were incorporated into the hydrogels and the cell viability and proliferation of cells were assessed. Results: In this study, a biomimetic injectable chitosan/pectin hydrogel was produced with excellent properties for CTTE scaffold. The hydrogel was optimized and showed suitable physicochemical properties, including thermal stability, compressive strength, viscoelastic behavior, swelling ratio, and degradation rate. Having such characteristics, the hydrogel meets the requirements for the cartilage repair. The hydrogel precursors and cross-linked hydrogels were thoroughly characterized regarding their chemical, morphological, microstructural and mechanical properties, as well as their swelling and degradation profiles. The hydrogels provided a host tissue-mimetic microenvironment for maintaining chondrocyte phenotype. Conclusion: Altogether, we successfully prepared the biomimetic injectable hydrogel without incorporating any extraneous cross-linking agents under physiological conditions. The properties of the hydrogel such as gelation time, mechanical properties and degradation behavior, were easily adjusted. When chondrocytes were encapsulated into hydrogels, it was found that cell behavior was remarkably affected by CS/pectin composition. The in-vitro study showed that the incorporation of chondrocytes to the hydrogel was able to maintain long-term chondrocytes survivability and improve cartilaginous ECM deposition. We believe the biomimetic injectable hydrogel based on polysaccharides is very promising scaffolds for CTTE application. |
| کلمات کلیدی | Injectable hydrogels; Adipose-derived stem cells; Cartilage tissue engineering |
| نام فایل | تاریخ درج فایل | اندازه فایل | دانلود |
|---|---|---|---|
| 11804-20191029-121274798040262.jpg | 1398/08/11 | 792290 | دانلود |
| bi-8-s1 (1).pdf | 1398/08/11 | 2905933 | دانلود |
